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通过对扩增的病毒核酸进行比色双寡核苷酸吸附测定法灵敏鉴定蓝舌病毒血清群

Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid.

作者信息

Katz J B, Alstad A D, Gustafson G A, Moser K M

机构信息

Diagnostic Virology Laboratory, U.S. Department of Agriculture, Ames, Iowa 50010.

出版信息

J Clin Microbiol. 1993 Nov;31(11):3028-30. doi: 10.1128/jcm.31.11.3028-3030.1993.

DOI:10.1128/jcm.31.11.3028-3030.1993
PMID:8263190
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266197/
Abstract

A polymerase chain reaction (PCR) procedure coupled to an enzyme-linked oligonucleotide sorbent assay (ELOSA; a PCR-ELOSA) identified all 24 serotypic variants of bluetongue virus (BTV) without identifying any of six viruses belonging to the related epizootic hemorrhagic disease virus serogroup. The PCR-ELOSA detected 0.01 50% cell culture infectious doses of each serotype of BTV. The sensitivity and serogroup-wide specificity of the PCR-ELOSA may enable it to replace the more expensive, time-consuming, and biohazardous methods used in the identification of BTV.

摘要

一种与酶联寡核苷酸吸附测定法（ELOSA；PCR-ELOSA）相结合的聚合酶链反应（PCR）程序，能够鉴定蓝舌病毒（BTV）的所有24种血清型变异体，而不会鉴定出属于相关流行性出血病病毒血清群的六种病毒中的任何一种。PCR-ELOSA检测到每种血清型BTV的0.01 50%细胞培养感染剂量。PCR-ELOSA的灵敏度和血清群范围内的特异性可能使其能够取代用于鉴定BTV的更昂贵、耗时且具有生物危害性的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ce9/266197/2a4fa47290b7/jcm00023-0203-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ce9/266197/49763fa2ab5f/jcm00023-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ce9/266197/2a4fa47290b7/jcm00023-0203-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ce9/266197/49763fa2ab5f/jcm00023-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ce9/266197/2a4fa47290b7/jcm00023-0203-b.jpg

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