Rao Pavuluri Panduranga, Reddy Y Vishnuvardhan, Meena Keerti, Karunasree N, Susmitha B, Uma Madala, Prasad P U V S, Chaitanya P, Reddy Y Narsimaha, Hegde Nagendra R
Ella Foundation, Genome Valley, Turkapally, Shameerpet Mandal, Hyderabad 500078, India.
Virus Genes. 2012 Apr;44(2):286-94. doi: 10.1007/s11262-011-0707-4. Epub 2012 Jan 19.
Recent incursions of bluetongue virus (BTV) into previously naive geographical areas have emphasised the need to better understand virus movement and epidemiology. Several bluetongue virus (BTV) serotypes are known to exist in India, and some serotype viruses have been isolated. However, the complete genome of not a single isolate is available to date. We report the complete genome sequence of one, and partial sequences of three other Indian isolates of BTV-9. Evolutionary relationships with segment-2 and -6 sequences of BTV isolates around the world, deduced using four different phylogenetic analyses and a similarity programme, show that BTV-9 (Eastern), BTV-9 (Western), and BTV-5 form a triad of equidistant, genetically distinct groups of viruses. The Indian BTV-9 isolates were closely related to Mediterranean and European BTV-9 isolates (Eastern topotype) based on segment-2 and -6 sequences. By contrast, segment-5 analyses clustered the Indian BTV-9 isolates with South African BTV-3 reference strain (98% identity), which belongs to one of the Western types. These results have implications on BTV origin and movement, genotyping, serotyping, and vaccine design.
最近蓝舌病病毒（BTV）侵入以前未受感染的地理区域，这凸显了更好地了解病毒传播和流行病学的必要性。已知印度存在几种蓝舌病病毒（BTV）血清型，并且已经分离出一些血清型病毒。然而，迄今为止尚未获得任何一个分离株的完整基因组。我们报告了一株BTV-9的完整基因组序列以及其他三株印度BTV-9分离株的部分序列。使用四种不同的系统发育分析和一个相似性程序推导得出，与世界各地BTV分离株的第2和第6片段序列的进化关系表明，BTV-9（东部）、BTV-9（西部）和BTV-5形成了一组等距、基因上不同的病毒群。基于第2和第6片段序列，印度BTV-9分离株与地中海和欧洲的BTV-9分离株（东部典型型）密切相关。相比之下，第5片段分析将印度BTV-9分离株与南非BTV-3参考毒株聚类在一起（同一性为98%），该毒株属于西部类型之一。这些结果对BTV的起源和传播、基因分型、血清分型以及疫苗设计具有重要意义。
