Ingman T, Sorsa T, Konttinen Y T, Liede K, Saari H, Lindy O, Suomalainen K
Department of Periodontology, University of Helsinki, Finland.
Oral Microbiol Immunol. 1993 Oct;8(5):298-305. doi: 10.1111/j.1399-302x.1993.tb00578.x.
The profile of salivary proteases and their cellular origin, with special reference to polymorphonuclear leukocytes and bacteria, was studied in localized juvenile periodontitis and compared with adult periodontitis and healthy controls. General proteolytic activity in saliva as well as collagenase, elastase-like and trypsin-like activity was measured. In addition, the sensitivity of salivary collagenase of patients with localized juvenile periodontitis to doxycycline inhibition was studied. The saliva of localized juvenile periodontitis patients contained low amounts of collagenase compared with adult periodontitis saliva, and almost all salivary collagenase was found to exist in endogenously active form, as was found to be the case also in adult periodontitis patients and healthy controls. The salivary collagenase of localized juvenile periodontitis patients was relatively insensitive to 100 mumol/l doxycycline but was completely inhibited by 600 mumol/l doxycycline, reflecting rather matrix metalloproteinase-1 (fibroblast-type) than matrix metalloproteinase-8 (polymorphonuclear leukocyte) enzyme. The saliva of localized juvenile periodontitis patients also contained low amounts of elastase-like activity compared with the saliva of untreated adult periodontitis patients. Scaling and root planing caused a significant decrease in elastase-like activity in the saliva of adult periodontitis patients. General proteolytic and trypsin-like activities were also low in the saliva of localized juvenile periodontitis patients. Furthermore, the reducing agent beta-mercaptoethanol did not activate or inhibit the salivary trypsin-like activity of localized juvenile periodontitis or adult periodontitis patients, although the reductant readily activated partially purified Porphyromonas gingivalis trypsin-like protease in a characteristic manner.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究针对局限性青少年牙周炎患者唾液中的蛋白酶及其细胞来源，特别关注多形核白细胞和细菌，并与成人牙周炎患者及健康对照进行比较。检测了唾液中的一般蛋白水解活性以及胶原酶、类弹性蛋白酶和类胰蛋白酶活性。此外，还研究了局限性青少年牙周炎患者唾液胶原酶对强力霉素抑制作用的敏感性。与成人牙周炎患者的唾液相比，局限性青少年牙周炎患者的唾液中胶原酶含量较低，并且几乎所有唾液胶原酶均以内源活性形式存在，成人牙周炎患者和健康对照者的情况也是如此。局限性青少年牙周炎患者的唾液胶原酶对100 μmol/L强力霉素相对不敏感，但600 μmol/L强力霉素可将其完全抑制，这表明该酶更像是基质金属蛋白酶-1（成纤维细胞型）而非基质金属蛋白酶-8（多形核白细胞型）。与未经治疗的成人牙周炎患者的唾液相比，局限性青少年牙周炎患者的唾液中类弹性蛋白酶活性也较低。龈下刮治和根面平整术可使成人牙周炎患者唾液中的类弹性蛋白酶活性显著降低。局限性青少年牙周炎患者唾液中的一般蛋白水解活性和类胰蛋白酶活性也较低。此外，还原剂β-巯基乙醇并未激活或抑制局限性青少年牙周炎或成人牙周炎患者唾液中的类胰蛋白酶活性，尽管该还原剂能以特征性方式轻易激活部分纯化的牙龈卟啉单胞菌类胰蛋白酶。（摘要截选至250词）
