Yukhananov R Y, Tennilä T M, Miroshnicenko T I, Kudrin V S, Ushakov A N, Melnik E I, Michaleva I I, Rayevsky K S, Airaksinen M M, Maisky A I
Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow.
Pharmacol Biochem Behav. 1992 Nov;43(3):683-7. doi: 10.1016/0091-3057(92)90396-w.
The brain content of dopamine (DA) and its metabolites [dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA)] were the same in rats with different immobilization times in forced swimming test, while the serotonin (5-HT) concentration was higher in high active (HA, immobilization < 2 min) than low active (LA, immobilization > 5 min) animals. Ethanol (2 g/kg, PO) tended to increase the DA level in the striatum and nucleus accumbens in LA rats and decrease the 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) concentration in HA rats. delta-Sleep-inducing peptide (DSIP) injection reduced the level of 5-HT in the medial prefrontal cortex (MFC) in both groups, did not affect the concentration of DA or DOPAC, but increased HVA in the striatum of HA rats. DSIP injected before ethanol administration augmented the ethanol effects on 5-HT in the MFC and attenuated the action of ethanol on 5-HIAA in the nucleus accumbens. A relationship between the different levels of voluntary alcohol consumption and sensitivity to stress among LA and HA rats and the differences in DA and 5-HT concentrations is suggested. The use of LA and HA rats in developing models for testing of stress-shielding compounds is also described.
在强迫游泳试验中，不同固定时间的大鼠脑内多巴胺（DA）及其代谢产物[二羟基苯乙酸（DOPAC）和高香草酸（HVA）]含量相同，而高活性（HA，固定时间<2分钟）大鼠的血清素（5-HT）浓度高于低活性（LA，固定时间>5分钟）大鼠。乙醇（2 g/kg，口服）倾向于增加LA大鼠纹状体和伏隔核中的DA水平，并降低HA大鼠中的5-HT和5-羟吲哚乙酸（5-HIAA）浓度。注射δ-睡眠诱导肽（DSIP）可降低两组内侧前额叶皮质（MFC）中的5-HT水平，不影响DA或DOPAC浓度，但可增加HA大鼠纹状体中的HVA。在乙醇给药前注射DSIP可增强乙醇对MFC中5-HT的作用，并减弱乙醇对伏隔核中5-HIAA的作用。提示LA和HA大鼠之间不同水平的自愿酒精消费与应激敏感性以及DA和5-HT浓度差异之间存在关联。还描述了使用LA和HA大鼠建立应激屏蔽化合物测试模型的情况。
