Akin F J, Snook M E, Severson R E, Chamberlain W J, Walters D B
J Natl Cancer Inst. 1976 Jul;57(1):191-5. doi: 10.1093/jnci/57.1.191.
Fraction F20, which in other studies was the most tumorigenic neutral fraction of cigarette smoke condensate (CSC), was separated by gel filtration chromatography into refined subfractions for identification of the polynuclear aromatic hydrocarbons (PAH) and for bioassay on mouse skin. Several hundred PAH were positively identified. Subfraction F55. containing most of the carcinogenic PAH as well as numerous unidentified components, was almost as tumorigenic to 7,12-dimethylbenze[a]anthracene (DMBA)-pretreated female outbred CD-1 mice as was F20. When F55 was separated into two parts, the first containing unidentified material (F55A) and the second containing the PAH (F55B), neither was significantly tumorigenic. F55B, combined with two other active fractions from the neutral and the acidic portions of CSC, exhibited a synergistic tumorigenic effect on DMBA-pretreated mice. The results supported the concept that the PAH in cigarette smoke must interact with other components in order to exert a tumorigenic effect.
在其他研究中，馏分F20是香烟烟雾冷凝物（CSC）中最具致瘤性的中性馏分，通过凝胶过滤色谱法将其分离成精制亚馏分，用于鉴定多环芳烃（PAH）并在小鼠皮肤上进行生物测定。已明确鉴定出数百种PAH。亚馏分F55含有大部分致癌PAH以及许多未鉴定的成分，对经7,12 - 二甲基苯并[a]蒽（DMBA）预处理的雌性远交CD - 1小鼠的致瘤性几乎与F20相同。当F55分为两部分时，第一部分含有未鉴定物质（F55A），第二部分含有PAH（F55B），两者均无明显致瘤性。F55B与来自CSC中性和酸性部分的其他两个活性馏分组合，对经DMBA预处理的小鼠表现出协同致瘤作用。结果支持了这样一种观点，即香烟烟雾中的PAH必须与其他成分相互作用才能发挥致瘤作用。
