[从大鼠肾脏中分离抗肝癌相关非组蛋白染色体蛋白的单克隆抗体]
[The isolation of monoclonal antibodies to hepatoma-associated nonhistone chromosomal proteins from rat kidneys].
作者信息
Grandilevskaia A B, Polyntsev D G, Kushner V P, Ivanov V A
出版信息
Tsitologiia. 1993;35(9):57-61.
PMID:8266582
Abstract
A monoclonal antibody (5D2) has been produced to a fraction of nonhistone proteins of kidney origin, associated with the rat hepatoma 27 and not obtained in normal liver. The 5D2 antibody was found to precipitate three proteins with molecular weights of 22-23 kDa. Indirect immunofluorescence studies on fixed hepatoma cell-preparations showed speckled nuclear staining patterns. In contrast, the 5D2 antibody was unreactive with intact hepatocytes.
摘要
已制备出一种单克隆抗体（5D2），它针对源自肾脏的一部分非组蛋白，该部分与大鼠肝癌27相关，在正常肝脏中未发现。发现5D2抗体可沉淀出三种分子量为22 - 23 kDa的蛋白质。对固定的肝癌细胞制剂进行的间接免疫荧光研究显示出斑点状核染色模式。相比之下，5D2抗体与完整的肝细胞无反应。
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