内毒素、白细胞介素-1β、白细胞介素-6或肿瘤坏死因子-α不会急性刺激分离出的小鼠子宫肌层收缩活性。

Endotoxin, interleukin-1 beta, interleukin-6, or tumor necrosis factor-alpha do not acutely stimulate isolated murine myometrial contractile activity.

作者信息

Oshiro B T, Monga M, Eriksen N L, Graham J M, Weisbrodt N W, Blanco J D

机构信息

Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School at Houston.

出版信息

Am J Obstet Gynecol. 1993 Dec;169(6):1424-7. doi: 10.1016/0002-9378(93)90412-c.

DOI:10.1016/0002-9378(93)90412-c

PMID:8267040

Abstract

OBJECTIVES

Endotoxin, interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha have been implicated in the pathogenesis of preterm labor, but their acute effect on myometrial contractile activity is unknown. The objective of this study was to determine their effect on isolated pregnant murine myometrial contractile activity.

STUDY DESIGN

Isometric contractions were measured in myometrium isolated from pregnancy day 18 Swiss-Webster mice. Frequency, duration, amplitude, and integrated area were compared before and after the addition of endotoxin (10(3) and 10(4) ng/ml) (n = 6), interleukin-1 beta (10 and 10 ng/ml) (n = 6), interleukin-6 (1 and 10 ng/ml) (n = 6), and tumor necrosis factor-alpha (1 and 10 ng/ml) (n = 6). Results were analyzed with the Wilcoxon rank-sum test.

RESULTS

The addition of endotoxin, interleukin-1 beta, interleukin-6, or tumor necrosis factor-alpha did not result in a change in the contractile activity of isolated pregnant murine myometrium compared with control.

CONCLUSION

Endotoxin, interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha do not acutely increase isolated murine myometrial contractile activity.

摘要

目的

内毒素、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α与早产的发病机制有关，但其对子宫肌层收缩活动的急性影响尚不清楚。本研究的目的是确定它们对分离的妊娠小鼠子宫肌层收缩活动的影响。

研究设计

测量从妊娠第18天的瑞士韦伯斯特小鼠分离出的子宫肌层的等长收缩。在添加内毒素（10³和10⁴ ng/ml）（n = 6）、白细胞介素-1β（10和10 ng/ml）（n = 6）、白细胞介素-6（1和10 ng/ml）（n = 6）和肿瘤坏死因子-α（1和10 ng/ml）（n = 6）之前和之后，比较频率、持续时间、幅度和积分面积。结果采用Wilcoxon秩和检验进行分析。