Oshiro B T, Monga M, Eriksen N L, Graham J M, Weisbrodt N W, Blanco J D
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School at Houston.
Am J Obstet Gynecol. 1993 Dec;169(6):1424-7. doi: 10.1016/0002-9378(93)90412-c.
Endotoxin, interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha have been implicated in the pathogenesis of preterm labor, but their acute effect on myometrial contractile activity is unknown. The objective of this study was to determine their effect on isolated pregnant murine myometrial contractile activity.
Isometric contractions were measured in myometrium isolated from pregnancy day 18 Swiss-Webster mice. Frequency, duration, amplitude, and integrated area were compared before and after the addition of endotoxin (10(3) and 10(4) ng/ml) (n = 6), interleukin-1 beta (10 and 10 ng/ml) (n = 6), interleukin-6 (1 and 10 ng/ml) (n = 6), and tumor necrosis factor-alpha (1 and 10 ng/ml) (n = 6). Results were analyzed with the Wilcoxon rank-sum test.
The addition of endotoxin, interleukin-1 beta, interleukin-6, or tumor necrosis factor-alpha did not result in a change in the contractile activity of isolated pregnant murine myometrium compared with control.
Endotoxin, interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha do not acutely increase isolated murine myometrial contractile activity.
内毒素、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α与早产的发病机制有关，但其对子宫肌层收缩活动的急性影响尚不清楚。本研究的目的是确定它们对分离的妊娠小鼠子宫肌层收缩活动的影响。
测量从妊娠第18天的瑞士韦伯斯特小鼠分离出的子宫肌层的等长收缩。在添加内毒素（10³和10⁴ ng/ml）（n = 6）、白细胞介素-1β（10和10 ng/ml）（n = 6）、白细胞介素-6（1和10 ng/ml）（n = 6）和肿瘤坏死因子-α（1和10 ng/ml）（n = 6）之前和之后，比较频率、持续时间、幅度和积分面积。结果采用Wilcoxon秩和检验进行分析。
与对照组相比，添加内毒素、白细胞介素-1β、白细胞介素-6或肿瘤坏死因子-α不会导致分离的妊娠小鼠子宫肌层收缩活动发生变化。
内毒素、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α不会急性增加分离的小鼠子宫肌层收缩活动。
