Nishihara T, Okahashi N, Ueda N
Department of Oral Science, National Institute of Health, Tokyo, Japan.
Biochem Biophys Res Commun. 1993 Dec 15;197(2):985-91. doi: 10.1006/bbrc.1993.2576.
Activins, members of a family of the transforming growth factor beta (TGF beta), are involved in the regulation of multiple biological events. We found a novel effect of activin A on hybridoma and myeloma cell lines. Activin A exhibited a cytotoxic effect on interleukin-6 (IL-6)-dependent B9 cells and induced a significant increase in the proportion of fragmented DNA. B9 cells exposed to activin A released high amounts of lactate dehydrogenase (LDH) and exhibited the typical ladder pattern of DNA fragmentation of apoptotic cells. IL-6 did not prevent apoptosis of B9 cells induced by activin A. The cytotoxicity of activin A to B9 cells was suppressed by follistatin. On the other hand, TGF beta showed no cytotoxic effect on B9 cells. These findings indicate that apoptosis induced by activin A could be one of the mechanisms to prevent uncontrolled cell growth.
激活素是转化生长因子β（TGF-β）家族的成员，参与多种生物学事件的调控。我们发现了激活素A对杂交瘤和骨髓瘤细胞系的一种新作用。激活素A对白介素-6（IL-6）依赖的B9细胞具有细胞毒性作用，并导致DNA片段化比例显著增加。暴露于激活素A的B9细胞释放大量乳酸脱氢酶（LDH），并呈现凋亡细胞典型的DNA片段化梯形图谱。IL-6不能阻止激活素A诱导的B9细胞凋亡。卵泡抑素可抑制激活素A对B9细胞的细胞毒性。另一方面，TGF-β对B9细胞无细胞毒性作用。这些发现表明，激活素A诱导的凋亡可能是防止细胞不受控制生长的机制之一。
