Chabouté M E, Chaubet N, Gigot C, Philipps G
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Biochimie. 1993;75(7):523-31. doi: 10.1016/0300-9084(93)90057-y.
The primary structure of the plant histone genes has been deduced from the comparison of the nucleotide sequences of 23 genes and 14 cDNAs from eight different species. These data confirmed the extreme conservation of histones H3 and H4 in plant and animal kingdoms. Histone H2B is more variable than H2A and the histone H1 is the less conserved histone. Some interesting observations concerning the non-conserved regions of H2A and H2B in their extended C- and N-terminal regions are reported. Only three plant histone genes were found to possess intervening sequences: one H1 gene and two H3.3 like genes. The most striking differences found between the two kingdoms are the absence from plant histone genes of the palindromic structure existing downstream of the animal genes and the fact that plant histone mRNAs are polyadenylated. This suggests that the post-transcriptional regulation of expression of histone genes is different in the two kingdoms. In plants the multiple copies of the histone genes are organized into multigenic families. In the complex genome of maize the multiple copies of the genes are highly dispersed on the genome.
通过比较来自八个不同物种的23个基因和14个cDNA的核苷酸序列，推断出了植物组蛋白基因的一级结构。这些数据证实了组蛋白H3和H4在植物和动物界中的极端保守性。组蛋白H2B比H2A更具变异性，而组蛋白H1是保守性最低的组蛋白。报告了一些关于H2A和H2B在其延伸的C端和N端区域非保守区的有趣观察结果。仅发现三个植物组蛋白基因具有间隔序列：一个H1基因和两个H3.3样基因。在两个界之间发现的最显著差异是植物组蛋白基因中不存在动物基因下游存在的回文结构，以及植物组蛋白mRNA是多聚腺苷酸化的这一事实。这表明组蛋白基因表达的转录后调控在两个界中是不同的。在植物中，组蛋白基因的多个拷贝被组织成多基因家族。在玉米的复杂基因组中，基因的多个拷贝在基因组上高度分散。
