Muzzarelli R A, Zucchini C, Ilari P, Pugnaloni A, Mattioli Belmonte M, Biagini G, Castaldini C
Institute of Biochemistry, Faculty of Medicine, University of Ancona, Italy.
Biomaterials. 1993 Oct;14(12):925-9. doi: 10.1016/0142-9612(93)90134-n.
Bone defects were surgically produced in the tibiae of rabbits and medicated with freeze-dried methylpyrrolidinone chitosan. Histological observations 60 d after surgery showed a considerable presence of neoformed bone tissue, as opposed to controls, originating from the pre-existing bone as well as from the periosteum. The cationic nature and the chelating ability of the methylpyrrolidinone chitosan apparently favoured mineralization. Endosteal-periosteal and bone marrow osteoblast-like precursors, stimulated by growth factors entrapped in the coagulum-polysaccharide mixture, gave rise to intramembranous bone formation. The ultrastructural examination evidenced that bone osteoid was followed by mineralization of the tissue.
在兔胫骨上通过手术制造骨缺损，并使用冻干的甲基吡咯烷酮壳聚糖进行治疗。术后60天的组织学观察显示，与对照组相比，有大量新形成的骨组织，这些骨组织源自既存骨以及骨膜。甲基吡咯烷酮壳聚糖的阳离子性质和螯合能力显然有利于矿化。凝结物 - 多糖混合物中所含生长因子刺激骨内膜 - 骨膜和骨髓中成骨细胞样前体细胞，从而形成膜内成骨。超微结构检查证明，骨类骨质之后是组织的矿化。
