Imai K, Nass S J, Olowson M, Balling R
Max-Planck-Institute for Immunobiology, Freiburg, Germany.
Mamm Genome. 1993;4(10):560-4. doi: 10.1007/BF00361385.
Tail kinks (tk) is a classical mouse skeletal mutation, located on Chromosome (Chr) 9. As the first step for the positional cloning of the tk gene, we have established a genetic map of a region surrounding the tk locus by generating a backcross segregating for tk. From this backcross, 1004 progeny were analyzed for the coat-color phenotype of the proximally located dilute (d) gene and for the distally flanking microsatellite marker, D9Mit12. Fifty-six recombinants between d and tk and 75 recombinants between tk and D9Mit12 were identified, completing a panel of 130 recombinants including one double recombinant. This panel allowed us to map five microsatellite loci as well as d and Mod-1 with respect to tk. We show that one of the microsatellite markers mapped, D9Mit9, does not recombine at all with tk in our backcross. This indicates that the D9Mit9 locus will serve as a good starting point for a chromosomal walk to the tk gene.
尾巴弯曲（tk）是一种经典的小鼠骨骼突变，位于9号染色体（Chr）上。作为tk基因定位克隆的第一步，我们通过产生一个针对tk进行回交分离的群体，建立了tk基因座周围区域的遗传图谱。从这个回交群体中，对1004个后代进行了近端定位的稀释（d）基因的毛色表型分析以及远端侧翼微卫星标记D9Mit12的分析。在d和tk之间鉴定出56个重组体，在tk和D9Mit12之间鉴定出75个重组体，从而完成了一个包含130个重组体的面板，其中包括一个双重组体。这个面板使我们能够相对于tk定位五个微卫星基因座以及d和Mod-1。我们表明，在我们的回交中，定位的微卫星标记之一D9Mit9与tk根本不发生重组。这表明D9Mit9基因座将作为向tk基因进行染色体步移的一个良好起点。
