多囊肾基因pcy在小鼠9号染色体上的遗传定位。

Genetic mapping of the polycystic kidney gene, pcy, on mouse chromosome 9.

作者信息

Nagao S, Watanabe T, Ogiso N, Marunouchi T, Takahashi H

机构信息

Laboratory Animal Center, Fujita Health University, Aichi, Japan.

出版信息

Biochem Genet. 1995 Dec;33(11-12):401-12. doi: 10.1007/BF00554598.

DOI:10.1007/BF00554598

PMID:8825940

Abstract

The murine polycystic kidney disease gene, pcy, is an autosomal recessive trait located on chromosome 9. To determine the genetic locus of pcy, 222 intraspecific backcross mice were obtained by mating C57BL/6FG-pcy and Mus molossinus. Restriction fragment length polymorphism analysis of 70 of the 222 backcross progeny showed that pcy, dilute coat color (d), and cholecystokinin (Cck) were located in the order d--pcy--Cck from the centromere. Simple sequence repeat length polymorphism analysis of DNA of all 222 backcross mice was carried out using four markers which were located near the central regions of d and Cck. One and eight recombinations were detected between D9Mit24 and pcy and between D9Mit16 and pcy, respectively. However, no recombinant was observed among pcy, D9Mit14, and D9Mit148. These findings strongly suggest that D9Mit14 and D9Mit148 are located near the pcy gene and are good markers for chromosomal walking to this gene.

摘要

小鼠多囊肾病基因pcy是位于9号染色体上的常染色体隐性性状。为了确定pcy的基因位点，通过将C57BL/6FG-pcy与家鼠杂交获得了222只种内回交小鼠。对222只回交后代中的70只进行限制性片段长度多态性分析，结果表明，从着丝粒起，pcy、稀释毛色（d）和胆囊收缩素（Cck）的排列顺序为d--pcy--Cck。使用位于d和Cck中心区域附近的四个标记，对所有222只回交小鼠的DNA进行简单序列重复长度多态性分析。在D9Mit24与pcy之间以及D9Mit16与pcy之间分别检测到1次和8次重组。然而，在pcy、D9Mit14和D9Mit148之间未观察到重组。这些发现强烈表明，D9Mit14和D9Mit148位于pcy基因附近，是用于该基因染色体步移的良好标记。