Becker K G, Swergold G D, Ozato K, Thayer R E
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Cancer Institute, NIH, Bethesda, MD 20892.
Hum Mol Genet. 1993 Oct;2(10):1697-702. doi: 10.1093/hmg/2.10.1697.
The first step of the currently favored model for the mechanism of transposition of the human LINE-1 element involves the synthesis of full length LINE-1 mRNA. Previous work demonstrated that the 5'-terminal 100 base pairs of the human LINE-1 element (L1Hs) has an important role in regulating it's expression. Here we report further deletion analysis revealing the presence of a cis regulatory element overlapping the region between base pairs +12 and +18. Oligonucleotides containing this sequence form a specific complex with a nuclear protein extracted from NTera2D1 and Jurkat cells, and with recombinant YY1 produced in E. coli. The complex is competed by YY1 binding sites found in other genes, and is ablated by anti-YY1 serum. These results suggest that YY1 is involved in the regulation of L1Hs transcription and therefore transposition.
目前备受青睐的人类LINE-1元件转座机制模型的第一步涉及全长LINE-1 mRNA的合成。先前的研究表明，人类LINE-1元件（L1Hs）的5'末端100个碱基对在调节其表达中起重要作用。在此，我们报告进一步的缺失分析，揭示存在一个与碱基对+12至+18之间区域重叠的顺式调节元件。含有该序列的寡核苷酸与从NTera2D1和Jurkat细胞中提取的核蛋白以及在大肠杆菌中产生的重组YY1形成特异性复合物。该复合物可被其他基因中发现的YY1结合位点竞争，并被抗YY1血清消除。这些结果表明YY1参与L1Hs转录的调节，进而参与转座过程。
