Bakal R S, Wright L L
Department of Anatomy, Boston University School of Medicine, Mass. 02118.
Dev Neurosci. 1993;15(1):22-6. doi: 10.1159/000111312.
Developmental neuron death is well described in sensory and sympathetic ganglia derived from the neural crest. In this study, nodose ganglia were removed from 2 litters of postnatal rats (male and female; 1, 3, 5, 9, and 14 days old) in order to determine whether postnatal neuron degeneration occurs in the nodose ganglia, which is derived from ectodermal placode. The ganglia were embedded in paraffin, sectioned and stained with methylene blue. Neuronal nuclei were counted at a magnification of 100 and diameters of nodose nuclei were traced at each age. There was a significant (p < 0.001) increase in the nuclear diameter of the nodose neurons of male and female rats from birth to postnatal day 14. In male rats, this difference was most marked between postnatal day 5 and postnatal day 14. The results of the neuron counts for both male and female rats indicated a gradual, significant (p < 0.001) decrease in the neuron population from day 1 to 14. For the males a 57.2% decline was observed, while the females displayed a 49.2% decline. The numbers of neurons in male and female ganglia showed no consistent differences. The data for neuron counts suggest that developmental neuron death occurs in postnatal rats with a gradual decrease in number of nodose neurons. However, since our findings show no evidence of degenerating nodose neurons, we are unable to rule out the possibility of migration from the developing ganglion.
发育性神经元死亡在源自神经嵴的感觉神经节和交感神经节中已有充分描述。在本研究中，从2窝新生大鼠（雄性和雌性；1、3、5、9和14日龄）中取出结节神经节，以确定源自外胚层基板的结节神经节在出生后是否发生神经元变性。将神经节包埋在石蜡中，切片并用亚甲蓝染色。在100倍放大倍数下计数神经元核，并在每个年龄追踪结节核的直径。从出生到出生后第14天，雄性和雌性大鼠结节神经元的核直径均有显著增加（p<0.001）。在雄性大鼠中，这种差异在出生后第5天和第14天之间最为明显。雄性和雌性大鼠的神经元计数结果表明，从第1天到第14天，神经元数量逐渐显著减少（p<0.001）。雄性大鼠观察到57.2%的下降，而雌性大鼠下降了49.2%。雄性和雌性神经节中的神经元数量没有一致的差异。神经元计数数据表明，发育性神经元死亡发生在新生大鼠中，结节神经元数量逐渐减少。然而，由于我们的研究结果没有显示结节神经元变性的证据，我们无法排除从发育中的神经节迁移的可能性。
