Greene A K, Few B K, Serafini J C
Department of Animal, Dairy and Veterinary Sciences, Clemson University, SC 29634.
J Dairy Sci. 1993 Nov;76(11):3617-20. doi: 10.3168/jds.S0022-0302(93)77702-4.
Ozonated water and chlorinated sanitizer were compared for effectiveness against biofilms of milk spoilage bacteria. Stainless steel plates were incubated in UHT-pasteurized milk inoculated with pure cultures of either Pseudomonas fluorescens (ATCC 949) or Alcaligenes faecalis (ATCC 337). After incubation, the plates were removed and rinsed in sterile PBS. A control rinsed stainless steel plate was swabbed and plated on standard plate count agar. A second rinsed stainless steel plate was covered and treated for 2 min with a commercial chlorinated sanitizer (dichloro-s-triazinetrione), prepared according to the manufacturer's recommendations; after treatment, the plate was rinsed twice in sterile PBS, swabbed, and plated on standard plate count agar. A third rinsed stainless steel plate from the culture was placed in ozonated deionized H2O (.5 ppm of ozone) for 10 min, rinsed twice as described, swabbed, and plated. Both ozonation and chlorination reduced bacteria populations by > 99% at initial cell densities in the range of approximately 1.24 x 10(5) to 8.56 x 10(5) cfu/cm2 for P. fluorescens and 1.53 x 10(4) to 8.56 x 10(5) cfu/cm2 for A. faecalis in milk films on stainless steel surfaces.
对臭氧水和含氯消毒剂针对牛奶变质细菌生物膜的有效性进行了比较。将不锈钢板置于接种了荧光假单胞菌（ATCC 949）或粪产碱菌（ATCC 337）纯培养物的超高温瞬时灭菌牛奶中进行培养。培养后，取出平板并在无菌磷酸盐缓冲盐水中冲洗。对一块冲洗过的不锈钢对照平板进行擦拭，并接种于标准平板计数琼脂上。将另一块冲洗过的不锈钢平板覆盖，并用按照制造商建议制备的商用含氯消毒剂（二氯-s-三嗪三酮）处理2分钟；处理后，将平板在无菌磷酸盐缓冲盐水中冲洗两次，擦拭后接种于标准平板计数琼脂上。将来自培养物的第三块冲洗过的不锈钢平板置于臭氧去离子水（臭氧浓度为0.5 ppm）中10分钟，按上述方法冲洗两次，擦拭后接种。对于不锈钢表面牛奶膜中荧光假单胞菌初始细胞密度约为1.24×10⁵至8.56×10⁵ cfu/cm²以及粪产碱菌初始细胞密度约为1.53×10⁴至8.56×10⁵ cfu/cm²的情况，臭氧处理和氯化处理均使细菌数量减少了99%以上。
