胰蛋白酶诱导的人血小板中的磷脂酶活性。
Trypsin-induced phospholipase activity in human platelets.
作者信息
Pickett W C, Jesse R L, Cohen P
出版信息
Biochem J. 1976 Nov 15;160(2):405-8. doi: 10.1042/bj1600405.
Abstract
Trypsin mediates a release of arachidonic acid with resultant increase in O2 consumption (a reflection of cyclo-oxygenase activity) by whole human platelets that is similar to thrombin's effect on these cells. The trypsin and thrombin effects can be differentiated in two ways: (1) at saturating concentrations the measured effects of trypsin greatly exceed those of thrombin; (2) EGTA [ethanedioxybis(ethylamine)-NNN'N'-tera-acetate] augments the effect of thrombin but not of trypsin. Thus trypsin and thrombin probably act at different loci in the pathway that induces phospholipase activity in human platelets.
摘要
胰蛋白酶介导花生四烯酸的释放，从而导致全血人血小板的耗氧量增加（这反映了环氧化酶的活性），这种作用类似于凝血酶对这些细胞的作用。胰蛋白酶和凝血酶的作用可以通过两种方式加以区分：（1）在饱和浓度下，所测得的胰蛋白酶的作用大大超过凝血酶的作用；（2）乙二醇双（乙胺）-NNN'N'-四乙酸（EGTA）增强凝血酶的作用，但不增强胰蛋白酶的作用。因此，胰蛋白酶和凝血酶可能作用于诱导人血小板中磷脂酶活性途径的不同位点。
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