von Deimling O H
Biochem Genet. 1984 Oct;22(9-10):769-82. doi: 10.1007/BF00499472.
Genetic variation of a carboxylesterase isozyme (EC 3.1.1.1) of the house mouse, designated ES-23, is described. ES-23 was found in kidney, liver, and intestine. The isozyme was resistant to inhibition by 10(-3) mol/liter eserine and was stained using alpha-naphthyl butyrate or 5-bromoindoxyl acetate as substrate. Five different phenotypes, ES-23A to ES-23E, could be distinguished by disc electrophoresis and by isoelectric focusing. ES-23 is controlled by a structural locus situated within the esterase gene cluster 2 on chromosome 8. An analysis of allele distribution among different strains suggested a separate structural locus for the isozyme, Es-23e, which is closely linked to the loci Es-2, Es-5, Es-7, and Es-11. Of the five phenotypes, only ES-23B was expressed in lung. This variation is apparently controlled by a cis-acting regulatory element, presumably a temporal locus, Es-23t, closely linked to the presumed structural locus Es-23e.
本文描述了小家鼠一种羧酸酯酶同工酶（EC 3.1.1.1）的遗传变异，该同工酶命名为ES - 23。ES - 23存在于肾脏、肝脏和肠道中。该同工酶对10⁻³摩尔/升毒扁豆碱的抑制作用具有抗性，并且以α - 萘基丁酸或5 - 溴吲哚乙酸作为底物进行染色。通过圆盘电泳和等电聚焦可区分出五种不同的表型，即ES - 23A至ES - 23E。ES - 23由位于8号染色体上酯酶基因簇2内的一个结构基因座控制。对不同品系中等位基因分布的分析表明，该同工酶存在一个单独的结构基因座Es - 23e，它与基因座Es - 2、Es - 5、Es - 7和Es - 11紧密连锁。在这五种表型中，只有ES - 23B在肺中表达。这种变异显然受一个顺式作用调节元件控制，推测是一个紧密连锁于假定结构基因座Es - 23e的时间基因座Es - 23t。
