Yan Y Z, Lin Q L, Xie P S
Guangzhou Municipal Institute for Drug Control.
Zhongguo Zhong Yao Za Zhi. 1993 Jun;18(6):329-31, 380.
This paper introduces the use of optimized solvent system with twice-ascending-development in twin trough chamber on TLC silica gel plate for the separation of protoberberine-type alkaloids contained in Rhizomata of Coptis spp. Nine to eleven spots including the main and the 'minor' alkaloids in the samples can be observed on the chromatograms obtained under controlled conditions. The fluorescence and UV-absorption TLC scanning profile can serve as fingerprint for the analysis of commercial samples of Rhizomata of Coptis spp.
本文介绍了在硅胶薄层板双槽展开室中使用优化溶剂系统进行二次升序展开，用于分离黄连属植物根茎中所含的原小檗碱型生物碱。在控制条件下获得的色谱图上可观察到样品中包括主要生物碱和“次要”生物碱在内的9至11个斑点。荧光和紫外吸收薄层扫描图谱可作为分析黄连属植物根茎商业样品的指纹图谱。
