Cabeza-Arvelaiz Y, Shih L C, Hardman N, Asselbergs F, Bilbe G, Schmitz A, White B, Siciliano M J, Lachman L B
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
DNA Cell Biol. 1993 Dec;12(10):881-92. doi: 10.1089/dna.1993.12.881.
We report the isolation, sequence, and identification of a cDNA encoding the human kinesin light-chain (KLC) protein. The cDNA molecule consisted of 276 nucleotides of 5' untranslated region, the complete coding sequence of 1,710 nucleotides, and 322 nucleotides of 3' untranslated region. It encoded a polypeptide of 569 amino acids and a deduced molecular mass of 64,789 daltons. The predicted secondary internal structure of the KLC molecule consisted of about 27 contiguous repeats, each of approximately 21 amino acid residues, and could be divided into three domains. The amino-terminal domain consisted of heptad repeats typical of the rod domain of several cytoskeletal proteins. The central and carboxy-terminal domains consist of 21-mer repeats. KLC mRNA was expressed in most tissues analyzed. The gene, which was expressed in bacteria and Chinese hamster ovary cells, was provisionally assigned to the long arm of human chromosome 14.
我们报告了编码人驱动蛋白轻链（KLC）蛋白的cDNA的分离、测序及鉴定。该cDNA分子由5'非翻译区的276个核苷酸、1710个核苷酸的完整编码序列以及3'非翻译区的322个核苷酸组成。它编码一个由569个氨基酸组成的多肽，推导分子量为64,789道尔顿。KLC分子预测的二级内部结构由约27个连续重复序列组成，每个重复序列约21个氨基酸残基，可分为三个结构域。氨基末端结构域由几种细胞骨架蛋白杆状结构域典型的七肽重复序列组成。中央和羧基末端结构域由21聚体重复序列组成。KLC mRNA在大多数分析的组织中表达。该基因在细菌和中国仓鼠卵巢细胞中表达，暂定位到人染色体14的长臂上。
