de la Torre M, Wells A F, Bergh J, Lindgren A
Department of Pathology, University Hospital of Uppsala, Sweden.
Hum Pathol. 1993 Dec;24(12):1294-7. doi: 10.1016/0046-8177(93)90262-f.
Hyaluronan (hyaluronic acid [HYA]) is one of the extracellular matrix components involved in normal cell physiology and is localized mainly in bodily fluids and connective tissues. Increased amounts of HYA in serum have been demonstrated in a number of neoplastic and inflammatory conditions, among them breast cancer. Tubular breast carcinoma (TC) and radial scar (RS) are two breast lesions that microscopically display characteristic stromal alterations and possess gross and microscopic similarities. Due to the importance of HYA as a component of the extracellular matrix, we investigated its presence in these lesions and in normal breast tissue. Using a biotinylated HYA-binding region for the in situ detection of HYA, we noted an increased amount of HYA in both TC and RS as compared with that in normal breast tissue specimens. A strong reactivity was observed predominantly around glandular structures and in the interlobular stroma of both TC and RS. Perivascular HYA staining also was distinctly observed in these lesions (TC and RS). Some HYA was observed in the connective tissue of the intralobular regions, around small blood vessels, and in the perivascular connective tissue of the normal breast. The distribution of HYA adjacent to the epithelium in the normal breast suggests a role for HYA in the interaction between epithelium and stroma of the normal breast. Its increase in the connective tissue of both TC and RS reflects the derangement of the stroma commonly observed in these conditions and supports the notion that these lesions may be associated.
透明质酸（玻尿酸[HYA]）是参与正常细胞生理过程的细胞外基质成分之一，主要定位于体液和结缔组织中。在许多肿瘤性和炎症性疾病中，包括乳腺癌，血清中透明质酸的含量都会增加。管状乳腺癌（TC）和放射状瘢痕（RS）是两种乳腺病变，在显微镜下显示出特征性的间质改变，并且在大体和显微镜下具有相似性。由于透明质酸作为细胞外基质成分的重要性，我们研究了其在这些病变以及正常乳腺组织中的存在情况。使用生物素化的透明质酸结合区域对透明质酸进行原位检测，我们发现与正常乳腺组织标本相比，TC和RS中的透明质酸含量均增加。在TC和RS的腺管结构周围以及小叶间质中均观察到强反应性。在这些病变（TC和RS）中也明显观察到血管周围透明质酸染色。在正常乳腺的小叶内区域的结缔组织、小血管周围以及血管周围结缔组织中也观察到一些透明质酸。正常乳腺中上皮附近透明质酸的分布表明透明质酸在正常乳腺上皮与间质之间的相互作用中发挥作用。其在TC和RS的结缔组织中的增加反映了在这些情况下常见的间质紊乱，并支持了这些病变可能相关的观点。
