Piehl-Aulin K, Laurent C, Engström-Laurent A, Hellström S, Henriksson J
Department of Rheumatology, Central Hospital, Falun, Sweden.
J Appl Physiol (1985). 1991 Dec;71(6):2493-8. doi: 10.1152/jappl.1991.71.6.2493.
The concentration and localization of hyaluronan (HYA) were determined in biopsy specimens from resting human quadriceps femoris and anterior tibial muscles. The influence of physical exercise on HYA concentrations in the quadriceps femoris muscle and in blood was also evaluated. A sensitive radioassay was used for the quantification of HYA. The distribution of the glycosaminoglycan was demonstrated using a histochemical method that involved microwave-aided fixation and an HYA-binding protein. At rest, the muscle HYA concentration was 34.9 +/- 23.6 (SD) micrograms/g muscle wet wt with a large interindividual variation. Exercise had no significant effect on the muscle HYA concentration. The serum HYA concentration increased from 35.9 +/- 22.7 to 53.4 +/- 57.1 micrograms/l during exercise, but 30 min after the exercise the HYA concentration was significantly lower (19.1 +/- 6.3 micrograms/l) than the initial preexercise value. In resting skeletal muscles of the lower extremity, HYA was heterogeneously distributed in the perimysium and endomysium. Perivascular and perineural connective tissues were distinctly HYA positive.
在取自静息状态下人类股四头肌和胫骨前肌的活检标本中测定了透明质酸（HYA）的浓度和定位。还评估了体育锻炼对股四头肌和血液中HYA浓度的影响。使用一种灵敏的放射测定法对HYA进行定量。采用一种组织化学方法显示糖胺聚糖的分布，该方法涉及微波辅助固定和一种HYA结合蛋白。静息时，肌肉HYA浓度为34.9±23.6（标准差）微克/克肌肉湿重，个体间差异较大。锻炼对肌肉HYA浓度无显著影响。运动期间血清HYA浓度从35.9±22.7微克/升增加至53.4±57.1微克/升，但运动后30分钟，HYA浓度显著低于运动前初始值（19.1±6.3微克/升）。在下肢静息骨骼肌中，HYA在肌束膜和肌内膜中呈异质性分布。血管周围和神经周围结缔组织明显呈HYA阳性。
