Laurent C, Johnson-Wells G, Hellström S, Engström-Laurent A, Wells A F
Department of Anatomy and Otorhinolaryngology, University of Umeå, Sweden.
Cell Tissue Res. 1991 Feb;263(2):201-5. doi: 10.1007/BF00318761.
The histochemical distribution of hyaluronan (hyaluronic acid, HYA) was analysed in various types of muscles in the rat by use of a hyaluronan-binding protein (HABP) and the avidin-biotin/peroxidase complex staining procedure. Microwave-aided fixation was used to retain the extracellular location of the glycosaminoglycan. In skeletal muscles, HYA was detected in the connective tissue sheath surrounding the muscles (epimysium), in the septa subdividing the muscle fibre bundles (perimysium) and in the connective tissue surrounding each muscle fibre (endomysium). HYA was heterogeneously distributed in all striated muscles. In skeletal muscles with small fibre dimensions (e.g., the lateral rectus muscle of the eye and the middle ear muscles), HYA was predominantly accumulated around the individual muscle fibres. Perivascular and perineural connective tissue formations were distinctly HYA-positive. In cardiac muscles, HYA was randomly distributed around the branching and interconnecting muscle fibres. In comparison, smooth muscle tissue was devoid of HYA.
利用透明质酸结合蛋白（HABP）和抗生物素蛋白-生物素/过氧化物酶复合物染色程序，分析了大鼠不同类型肌肉中透明质酸（HYA）的组织化学分布。采用微波辅助固定以保留糖胺聚糖的细胞外位置。在骨骼肌中，在围绕肌肉的结缔组织鞘（肌外膜）、分隔肌纤维束的间隔（肌束膜）以及围绕每条肌纤维的结缔组织（肌内膜）中检测到HYA。HYA在所有横纹肌中呈异质性分布。在纤维尺寸较小的骨骼肌（如眼外直肌和中耳肌肉）中，HYA主要积聚在单个肌纤维周围。血管周围和神经周围的结缔组织明显呈HYA阳性。在心肌中，HYA随机分布在分支和相互连接的肌纤维周围。相比之下，平滑肌组织中没有HYA。
