Chakrabarti B K, Chattopadhyay D J, Ghosh A N
Department of Biochemistry, University College of Science, Calcutta, India.
J Gen Virol. 1993 Dec;74 ( Pt 12):2749-52. doi: 10.1099/0022-1317-74-12-2749.
Phage D10, a Vibrio cholerae O-1 El Tor group X phage, is one of the five newly isolated phages used in the phage typing scheme developed for V. cholerae O-1 biotype El Tor and belongs to the Myoviridae family. From electron microscopic studies it is shown that phage D10 has a DNA genome of 32 +/- 0.2 kb. This is the first report where it has been shown by the construction of a partial denaturation map that this vibriophage genome is nonpermuted and has cohesive ends. The location of the ends of the DNA in the phage head has also been inferred.
噬菌体D10是霍乱弧菌O-1埃尔托型X群噬菌体，是为霍乱弧菌O-1生物型埃尔托型开发的噬菌体分型方案中使用的五种新分离的噬菌体之一，属于肌尾噬菌体科。电子显微镜研究表明，噬菌体D10的DNA基因组大小为32±0.2kb。这是首次通过构建部分变性图谱证明该弧菌噬菌体基因组是未重排的且具有粘性末端。还推断出了噬菌体头部DNA末端的位置。
