Raitt D C, Bradshaw R E, Pillar T M
Leicester Biocentre, Leicester University, UK.
Mol Gen Genet. 1994 Jan;242(1):17-22. doi: 10.1007/BF00277343.
The cytochrome c gene (cycA) of the filamentous fungus Aspergillus nidulans has been isolated and sequenced. The gene is present in a single copy per haploid genome and encodes a polypeptide of 112 amino acid residues. The nucleotide sequence of the A. nidulans cycA gene shows 87% identity to the DNA sequence of the Neurospora crassa cytochrome c gene, and approximately 72% identity to the sequence of the Saccharomyces cerevisiae iso-1-cytochrome c gene (CYC1). The S. cerevisiae CYC1 gene was used as a heterologous probe to isolate the homologous gene in A. nidulans. The A. nidulans cytochrome c sequence contains two small introns. One of these is highly conserved in terms of position, but the other has not been reported in any of the cytochrome c genes so far sequenced. Expression of the cycA gene is not affected by glucose repression, but has been shown to be induced approximately tenfold in the presence of oxygen and three- to fourfold under heat-shock conditions.
丝状真菌构巢曲霉的细胞色素c基因（cycA）已被分离并测序。该基因在每个单倍体基因组中以单拷贝形式存在，编码一个由112个氨基酸残基组成的多肽。构巢曲霉cycA基因的核苷酸序列与粗糙脉孢菌细胞色素c基因的DNA序列有87%的同一性，与酿酒酵母同工酶-1-细胞色素c基因（CYC1）的序列有大约72%的同一性。酿酒酵母CYC1基因被用作异源探针来分离构巢曲霉中的同源基因。构巢曲霉细胞色素c序列包含两个小内含子。其中一个在位置上高度保守，但另一个在迄今测序的任何细胞色素c基因中均未报道。cycA基因的表达不受葡萄糖阻遏的影响，但已证明在有氧存在时可被诱导约10倍，在热休克条件下可被诱导3至4倍。
