Limbach K J, Wu R
Nucleic Acids Res. 1983 Dec 20;11(24):8931-50. doi: 10.1093/nar/11.24.8931.
Analysis of total chicken DNA by genomic blot hybridization indicates that only one cytochrome c gene exists in the chicken genome. The two alleles of this single cytochrome c gene have been isolated from a Charon 4A-chicken genomic library. This isolation made use of the yeast CYC1 cytochrome c gene as a specific hybridization probe. The 2 chicken alleles, CC9 and CC10, have been sequenced. The amino acid sequence predicted by these 2 alleles is identical, and agrees with the published chicken cytochrome c protein sequence. The flanking regions of these 2 alleles exhibit approximately 1% divergence, indicating a very limited polymorphism. Comparative sequence analysis with the flanking regions of previously isolated cytochrome c genes (yeast and rat) indicate no significant regions of homology. The presence of only one cytochrome c-like sequence in the chicken genome is in striking contrast with mammalian genomes, which contain as many as 20-30 cytochrome c-like sequences.
通过基因组印迹杂交对鸡的总DNA进行分析表明，鸡基因组中仅存在一个细胞色素c基因。该单个细胞色素c基因的两个等位基因已从Charon 4A-鸡基因组文库中分离出来。这种分离利用酵母CYC1细胞色素c基因作为特异性杂交探针。已对这两个鸡等位基因CC9和CC10进行了测序。这两个等位基因预测的氨基酸序列相同，并且与已发表的鸡细胞色素c蛋白序列一致。这两个等位基因的侧翼区域表现出约1%的差异，表明多态性非常有限。与先前分离的细胞色素c基因（酵母和大鼠）的侧翼区域进行比较序列分析表明，没有明显的同源区域。鸡基因组中仅存在一个细胞色素c样序列，这与哺乳动物基因组形成了鲜明对比，哺乳动物基因组中含有多达20-30个细胞色素c样序列。
