Lockwood D H, Neu R L
Vivigen, Inc., Santa Fe, NM 87505.
Prenat Diagn. 1993 Sep;13(9):801-5. doi: 10.1002/pd.1970130903.
Our laboratory has received 1375 early amniotic fluid (EA) specimens during the past 5-year period for cytogenetics analysis. The gestational ages of the EA specimens were less than 14 weeks as estimated by ultrasound. The average volume of specimen received was 16 ml. Specimens were typically received in two collection tubes and cultured in Chang A and in supplemented MEM media using the in situ technique. Of the 1375 EA specimens received, 1356 were successfully cultured and yielded results. Abnormal results were found in 67 (4.9 per cent) of the cases. Nineteen specimens (1.4 per cent) failed to yield a result. The mean turn-around time (TAT) for all EA specimens was 8.28 days. In 1991, the average TAT for the EA specimens was 8.00 days compared with a TAT of 6.59 days for all specimens received over 14 weeks gestational age. The number of EA specimens received has increased from 1.5 per month in 1986 to 57 per month in 1991. In summary, our experience with EA specimens for cytogenetic analysis has demonstrated that the success rate is 98.6 per cent and that an increasing number of obstetricians are performing early amniocentesis as they seek to provide their patients with earlier results and an alternative to chorionic villus sampling.
在过去5年中，我们实验室接收了1375份早期羊水（EA）标本用于细胞遗传学分析。根据超声估计，EA标本的孕周小于14周。接收的标本平均体积为16毫升。标本通常收集在两个采集管中，采用原位技术在Chang A和补充的MEM培养基中培养。在收到的1375份EA标本中，1356份成功培养并得出结果。67例（4.9%）病例发现异常结果。19份标本（1.4%）未能得出结果。所有EA标本的平均周转时间（TAT）为8.28天。1991年，EA标本的平均TAT为8.00天，而孕周超过14周的所有标本的TAT为6.59天。接收的EA标本数量已从1986年的每月1.5份增加到1991年的每月57份。总之，我们对EA标本进行细胞遗传学分析的经验表明，成功率为98.6%，并且越来越多的产科医生在进行早期羊膜腔穿刺术，因为他们试图为患者提供更早的结果以及绒毛取样的替代方法。
