Duenas-Barajas E, Bernal J E, Vaught D R, Nerurkar V R, Sarmiento P, Yanagihara R, Gajdusek D C
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland.
Am J Trop Med Hyg. 1993 Dec;49(6):657-63. doi: 10.4269/ajtmh.1993.49.657.
The coexistence of infection with human T lymphotropic virus types I and II (HTLV-I and HTLV-II) has been demonstrated recently among the Wayuu Indians from the Guajira region of Colombia. To ascertain if other Indian groups in Colombia are similarly infected, we tested 1,250 sera, collected between 1990 and 1992 from 18 culturally distinct Amerindian tribes living in widely separated regions, for IgG antibodies against HTLV-I/II using enzyme-linked immunosorbent assay (ELISA) and Western blot. Sera were also tested for antibodies against human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) to investigate the overall burden of retrovirus infection in these semi-isolated indigenous groups. A total of 33 of the 1,250 samples were repeatedly reactive to HTLV-I/II antigens by ELISA, and of these, three sera from Waunana/Noanama Indians from the Choco area and two sera from Tunebo Indians from the Santander region were found to be infected with HTLV-I and HTLV-II, respectively, as verified by Western blot and differential ELISA. Thus, despite the small sample size, the overall seroprevalences for HTLV-I and HTLV-II infection among the Waunana/Noanama and Tunebo Indians were 2.1% and 5.0%, respectively. In contrast, none of the 29 Indians who exhibited reactivity to HIV-1/2 by ELISA were seropositive by Western blot. This study adds the Tunebo to the expanding list of Amerindian groups with high prevalences of HTLV-II infection. Further intensive investigations of such indigenous populations will clarify the natural history and disease potential of HTLV-II infection.
最近已证实，哥伦比亚瓜希拉地区的瓦尤印第安人同时感染了人类嗜T淋巴细胞病毒I型和II型（HTLV-I和HTLV-II）。为确定哥伦比亚的其他印第安群体是否也受到类似感染，我们使用酶联免疫吸附测定（ELISA）和蛋白质印迹法，检测了1990年至1992年间从生活在广泛分散地区的18个文化各异的美洲印第安部落收集的1250份血清中针对HTLV-I/II的IgG抗体。还检测了血清中针对人类免疫缺陷病毒1型和2型（HIV-1和HIV-2）的抗体，以调查这些半隔离的土著群体中逆转录病毒感染的总体负担。1250份样本中共有33份通过ELISA对HTLV-I/II抗原呈反复反应性，其中，来自乔科地区的瓦纳纳/诺阿纳马印第安人的3份血清和来自桑坦德地区的图内博印第安人的2份血清，经蛋白质印迹法和差异ELISA验证，分别感染了HTLV-I和HTLV-II。因此，尽管样本量较小，但瓦纳纳/诺阿纳马印第安人和图内博印第安人中HTLV-I和HTLV-II感染的总体血清阳性率分别为2.1%和5.0%。相比之下，ELISA检测对HIV-1/2呈反应性的29名印第安人中，蛋白质印迹法检测均未呈血清阳性。这项研究将图内博印第安人添加到HTLV-II感染患病率较高的美洲印第安群体不断增加的名单中。对此类土著人群进行进一步深入调查，将阐明HTLV-II感染的自然史和疾病潜力。
