Yamamoto S, Mita E, Nakatake H, Takimoto M, Koshy R, Matsubara K
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Biochem Biophys Res Commun. 1993 Dec 30;197(3):1209-15. doi: 10.1006/bbrc.1993.2605.
HBV DNAs are often found in integrated forms in human hepatocellular carcinoma (HCC). Discovery of a transactivation function coded by a limited region of the HBV genome has promoted us to survey our collection of HBV integrants with flanking cellular sequences, asking whether they might exhibit a transactivation function. In transient cotransfection assays using the HepG2 cell line, six out of the twelve integrants showed transactivation effects on the expression of cellular genes such as c-fos. These results strongly demonstrate that the transactivating effects of integrated HBV DNA are widely distributed, and some of these effects might be correlated to hepatocarcinogenesis.
乙肝病毒脱氧核糖核酸（HBV DNA）常以整合形式存在于人类肝细胞癌（HCC）中。乙肝病毒基因组有限区域编码的反式激活功能的发现促使我们研究收集到的带有侧翼细胞序列的乙肝病毒整合体，探究它们是否可能具有反式激活功能。在使用HepG2细胞系的瞬时共转染试验中，12个整合体中有6个对c-fos等细胞基因的表达表现出反式激活作用。这些结果有力地证明，整合的乙肝病毒脱氧核糖核酸的反式激活作用广泛存在，其中一些作用可能与肝癌发生有关。
