整合的乙型肝炎病毒DNA对细胞启动子的反式激活作用

Transactivation of cellular promoters by an integrated hepatitis B virus DNA.

作者信息

Yamamoto S, Nakatake H, Kawamoto S, Takimoto M, Koshy R, Matsubara K

机构信息

Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.

出版信息

Biochem Biophys Res Commun. 1993 Apr 15;192(1):111-8. doi: 10.1006/bbrc.1993.1388.

DOI:10.1006/bbrc.1993.1388

PMID:8476412

Abstract

A new Hepatitis B virus(HBV) DNA integrant clone DA2-6, isolated from a human hepatocellular carcinoma(HCC) genomic library, was tested for its ability to transactivate expression of other genes. DA2-6 consists of 3.7 kb flanking cellular sequences and an integrated 2.8 kb HBV DNA which covers the region of preS, S, and the 3' truncated X. Using a chloramphenicol acetyltransferase (CAT) assay, a number of cellular and viral promoters were transactivated by DA2-6, and the spectrum of transactivational effect was the same as that by the wild type X gene of the virus. Deletion mutant analyses indicated that the transactivation function of DA2-6 is expressed by the region that encodes a truncated X-cell fusion product.

摘要

从人肝细胞癌（HCC）基因组文库中分离出一种新的乙型肝炎病毒（HBV）DNA整合克隆DA2-6，并检测了其反式激活其他基因表达的能力。DA2-6由3.7 kb的侧翼细胞序列和一个整合的2.8 kb HBV DNA组成，该DNA覆盖前S、S和3'截短的X区域。使用氯霉素乙酰转移酶（CAT）分析，DA2-6反式激活了许多细胞和病毒启动子，反式激活作用的谱与病毒野生型X基因相同。缺失突变分析表明，DA2-6的反式激活功能由编码截短的X-细胞融合产物的区域表达。