Brash J L, Ten Hove P
Department of Chemical Engineering and Pathology, McMaster University, Hamilton, Ontario, Canada.
J Biomater Sci Polym Ed. 1993;4(6):591-9. doi: 10.1163/156856293x00230.
Adsorption of fibrinogen from buffer as a single protein and from plasma to four materials has been studied. The two NIH-NHLBI primary reference standards, filler free polydimethylsiloxane and low density polyethylene, were used along with polyvinylcholoride and cellulose materials supplied by the IUPAC Working Party. The materials were examined in both film and tubing form, except for polydimethylsiloxane which was studied only in tubing form. Adsorption was measured at room temperature using 125I-labelled fibrinogen. The order of adsorbed amounts in the single protein experiments was found to be: cellulose < PVC < PE = PDMS. Apparent adsorption affinities are in the same order. In plasma, all surfaces except cellulose showed maxima in adsorption as a function of plasma concentration after 5 min contact. This is indicative of initial adsorption followed by displacement of fibrinogen (the Vroman effect). Cellulose showed very low adsorption of fibrinogen from plasma. The Vroman maxima were more pronounced on the tubing samples than on the films, and, as for the single protein experiments, adsorption was found to be less on tubing than on film samples. A tentative interpretation of the Vroman effect data suggests that the order of procoagulant activity of the materials may be: PDMS = PE < PVC < cellulose.
对四种材料从缓冲液中作为单一蛋白质以及从血浆中吸附纤维蛋白原的情况进行了研究。使用了两种美国国立卫生研究院 - 国立心肺血液研究所的主要参考标准材料，即无填料聚二甲基硅氧烷和低密度聚乙烯，以及国际纯粹与应用化学联合会工作组提供的聚氯乙烯和纤维素材料。除了仅以管材形式研究的聚二甲基硅氧烷外，其他材料均以薄膜和管材两种形式进行了检测。在室温下使用125I标记的纤维蛋白原测量吸附情况。在单一蛋白质实验中，发现吸附量的顺序为：纤维素＜聚氯乙烯＜聚乙烯 = 聚二甲基硅氧烷。表观吸附亲和力也呈相同顺序。在血浆中，接触5分钟后，除纤维素外的所有表面的吸附量均随血浆浓度呈现最大值。这表明最初发生吸附，随后纤维蛋白原被置换（弗罗曼效应）。纤维素从血浆中吸附纤维蛋白原的量非常低。弗罗曼最大值在管材样品上比在薄膜上更明显，并且与单一蛋白质实验一样，发现管材上的吸附量比薄膜样品上的少。对弗罗曼效应数据的初步解释表明，材料的促凝血活性顺序可能为：聚二甲基硅氧烷 = 聚乙烯＜聚氯乙烯＜纤维素。
