Caix J, Janvier G, Legault B, Bordenave L, Rouais F, Basse-Cathalinat B, Baquey C
Inserm U. 306, Université de Bordeaux II, France.
J Biomater Sci Polym Ed. 1994;5(4):279-91. doi: 10.1163/156856294x00022.
Factors determining the thrombogenic response to particular artificial surfaces were investigated ex vivo in a canine shunt model. Methods using radioisotopic tracers made it possible to dynamically monitor the deposition of labelled blood cells and proteins on a NHLBI.DTB primary reference material polydimethylsiloxane (PRM.PDMS) and on a IUPAC reference material polyvinyl chloride (IUPAC.PVC). On the one hand, leukocyte affinity tau s(leu) (number of deposited leukocytes mm-2s-1) was not significantly different between IUPAC.PVC (tau s(leu) = 1.2-2.5) and PRM.PDMS (tau s(leu) = 1.5-3.4) and the fibrinogen adsorption rate varied from 33 to 48.10(-5) micrograms mm-2s-1 for both these materials. On the other hand, platelet affinity tau s(plat) (number of deposited platelets mm-2s-1) was significantly different (p < 0.05) for IUPAC.PVC and PRM.PDMS (tau s(plat)PVC = 683 +/- 200 > tau s(plat)PDMS = 327 +/- 80). Scanning electron micrographs of adherent platelets, red cells and leukocytes after blood contact ex vivo were performed after each experiment. This preliminary work contributes not only to quantify the adsorption of different radiotracers, but also to evaluate the superficial distribution of the labelled biological species on the inner surface of the tested biomaterials.
在犬类分流模型中对体外特定人工表面血栓形成反应的决定因素进行了研究。使用放射性同位素示踪剂的方法能够动态监测标记血细胞和蛋白质在国立心、肺和血液研究所（NHLBI）DTB一级参考材料聚二甲基硅氧烷（PRM.PDMS）和国际纯粹与应用化学联合会（IUPAC）参考材料聚氯乙烯（IUPAC.PVC）上的沉积情况。一方面，IUPAC.PVC（τs(leu)=1.2 - 2.5）和PRM.PDMS（τs(leu)=1.5 - 3.4）之间的白细胞亲和力τs(leu)（沉积白细胞数量mm-2s-1）无显著差异，且这两种材料的纤维蛋白原吸附率在33至48×10(-5)微克mm-2s-1之间变化。另一方面，IUPAC.PVC和PRM.PDMS的血小板亲和力τs(plat)（沉积血小板数量mm-2s-1）存在显著差异（p<0.05）（τs(plat)PVC = 683±200 > τs(plat)PDMS = 327±80）。每次实验后对体外血液接触后粘附的血小板、红细胞和白细胞进行扫描电子显微镜成像。这项初步工作不仅有助于量化不同放射性示踪剂的吸附，还能评估标记生物物种在受试生物材料内表面的表面分布情况。
