Nilius M, Ströhle A, Bode G, Malfertheiner P
Dept. of Internal Medicine, University of Bonn, Germany.
Zentralbl Bakteriol. 1993 Sep;280(1-2):259-72. doi: 10.1016/s0934-8840(11)80964-3.
In this study we attempted to transform "helical" forms of Helicobacter pylori to "coccoid like forms" (CLF) by induction with the following substances in vitro: bismuth subcitrate, bismuth subsalicylate, ampicillin, amoxicillin, erythromycin, ursodeoxycholic acid and glycochenodeoxycholic acid. Some liquid cultures were incubated for 24 days to induce CLF by aging. Changes in the protein pattern, urease enzyme activity and in the serological response against specific antigens were investigated. In all strains a significant but strain-variable rate of CLF was detected by induction of the tested substances. Beta-lactams and erythromycin generated a population of nearly 100% CLF, including many "spheroblasts". Relative induction rates by these substances were in the following order: beta-lactams and erythromycin > bismuth subcitrate > bismuth subsalicylate > bile acids. Strain variable reaction also was true for both inhibition of urease activity and influence on immunological response. Urease activity was lost in CLF induced by aging and was inhibited by bismuth salts. CLF induced by aging showed a loss of reactivity bands in the immunoblot. They always lost a 160 kD band and depending on the strain, 115 kD, 108 kD, 100 kD and 95 kD bands. Immunological response to the 120 kD band was reduced. Ultrastructural studies showed great degenerative changes of the cell wall in CLF induced by antibiotics but only few in CLF induced by bismuth salts and bile acids.
在本研究中，我们试图通过在体外使用以下物质诱导，将幽门螺杆菌的“螺旋形”形态转化为“类球形形态”（CLF）：枸橼酸铋、碱式水杨酸铋、氨苄西林、阿莫西林、红霉素、熊去氧胆酸和甘氨鹅去氧胆酸。一些液体培养物通过老化培养24天以诱导CLF。研究了蛋白质模式、脲酶活性以及针对特定抗原的血清学反应的变化。在所有菌株中，通过测试物质的诱导检测到显著但因菌株而异的CLF发生率。β-内酰胺类抗生素和红霉素诱导产生了近100%的CLF群体，包括许多“成球细胞”。这些物质的相对诱导率顺序如下：β-内酰胺类抗生素和红霉素＞枸橼酸铋＞碱式水杨酸铋＞胆汁酸。对于脲酶活性的抑制和对免疫反应的影响，菌株的反应也存在差异。老化诱导的CLF中脲酶活性丧失，铋盐可抑制脲酶活性。老化诱导的CLF在免疫印迹中显示反应带丢失。它们总是丢失一条160 kD的条带，并且根据菌株不同，还会丢失115 kD、108 kD、100 kD和95 kD的条带。对120 kD条带的免疫反应降低。超微结构研究表明，抗生素诱导的CLF中细胞壁有很大的退行性变化，而铋盐和胆汁酸诱导的CLF中则很少。
