19个X连锁EST的区域定位

Regional assignment of 19 X-linked ESTs.

作者信息

Parrish J E, Nelson D L

机构信息

Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.

出版信息

Hum Mol Genet. 1993 Nov;2(11):1901-5. doi: 10.1093/hmg/2.11.1901.

DOI:10.1093/hmg/2.11.1901

PMID:8281154

Abstract

Subchromosomal localizations for 19 X-linked expressed sequence tags (ESTs) have been determined. Two ESTs are located in Xq28, adding two novel genes to this disease-rich region. The remaining ESTs are located primarily in the pericentromeric region, with most mapping to Xp11.1-p21.1. YAC and cosmid genomic clones have been isolated for several of these loci. Available cDNAs have been used to characterize the corresponding transcripts by Northern analysis in multiple human tissues.

摘要

已确定19个X连锁表达序列标签（EST）的亚染色体定位。两个EST位于Xq28，给这个疾病富集区域增加了两个新基因。其余的EST主要位于着丝粒周围区域，大多数定位于Xp11.1-p21.1。已分离出其中几个位点的酵母人工染色体（YAC）和黏粒基因组克隆。利用现有的cDNA，通过对多种人体组织进行Northern分析来鉴定相应的转录本。