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通过富勒土法或酶法测定肌酐,对小鼠尿液适用,但对小鼠血浆不适用。

Creatinine assay by the fuller's earth procedure or by enzymatic determination is adequate for urine but not plasma of mice.

作者信息

Meyer M H, Smith S, Meyer R A

机构信息

Baxter Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232-2861.

出版信息

Comp Biochem Physiol B. 1993 Nov;106(3):685-9. doi: 10.1016/0305-0491(93)90150-4.

DOI:10.1016/0305-0491(93)90150-4
PMID:8281763
Abstract
  1. Mouse plasma creatinine measured by fuller's earth (15.1 +/- 0.5(41) microM, mean +/- SE) exceeded the same pools read by HPLC [7.5 +/- 0.2(41); P < 0.001]. 2. For mouse urine, the fuller's earth method (3.26 +/- 0.21 mM) slightly exceeded the HPLC method (2.65 +/- 0.18; P < 0.001) with good correlation (r = 0.915, P < 0.001). 3. An enzymatic method (creatinine PAP, Boehringer-Mannheim Diagnostics) for plasma creatinine (5.2 +/- 0.3 microM) was equivalent to HPLC (6.1 +/- 0.1, NS) but with poor correlation (r = 0.23, NS). 4. Creatinine in the urine agreed closely between HPLC (2.84 +/- 0.12 mM) and the enzymatic procedure (2.95 +/- 0.17, NS) with good correlation (r = 0.845, P < 0.001).
摘要
  1. 用富勒土法测得的小鼠血浆肌酐(15.1±0.5(41)微摩尔,均值±标准误)超过了用高效液相色谱法测得的同一组数据[7.5±0.2(41);P<0.001]。2. 对于小鼠尿液,富勒土法(3.26±0.21毫摩尔)略高于高效液相色谱法(2.65±0.18;P<0.001),且相关性良好(r = 0.915,P<0.001)。3. 一种用于血浆肌酐的酶法(肌酐PAP,勃林格殷格翰诊断公司)测得的值为(5.2±0.3微摩尔),与高效液相色谱法(6.1±0.1,无显著差异)相当,但相关性较差(r = 0.23,无显著差异)。4. 尿液中的肌酐在高效液相色谱法(2.84±0.12毫摩尔)和酶法(2.95±0.17,无显著差异)之间吻合度高,且相关性良好(r = 0.845,P<0.001)。

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1
Creatinine assay by the fuller's earth procedure or by enzymatic determination is adequate for urine but not plasma of mice.通过富勒土法或酶法测定肌酐,对小鼠尿液适用,但对小鼠血浆不适用。
Comp Biochem Physiol B. 1993 Nov;106(3):685-9. doi: 10.1016/0305-0491(93)90150-4.
2
Adsorption of creatinine to Fuller's earth.肌酐在富勒土上的吸附作用。
Scand J Clin Lab Invest. 1990 Oct;50(6):687-92. doi: 10.3109/00365519009089188.
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Assay of creatinine in serum, with use of fuller's earth to remove interferents.
Clin Chem. 1981 Jan;27(1):179-83.
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Reference values of serum and urine creatinine, and of creatinine clearance by a new enzymatic method.血清和尿液肌酐的参考值,以及采用一种新酶法测定的肌酐清除率
Ann Clin Biochem. 1992 Sep;29 ( Pt 5):523-8. doi: 10.1177/000456329202900506.
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Simplified determinations of the "true" creatinine concentration in serum and urine.血清和尿液中“真实”肌酐浓度的简化测定方法。
J Clin Chem Clin Biochem. 1980 Jul;18(7):385-94. doi: 10.1515/cclm.1980.18.7.385.
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Determination of creatinine in serum and urine by a rapid liquid-chromatographic method.
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Activated charcoal is as effective as fuller's earth or bentonite in paraquat poisoning.在百草枯中毒方面,活性炭与漂白土或膨润土的效果相同。
Klin Wochenschr. 1982 Feb 15;60(4):207-10. doi: 10.1007/BF01715588.
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Thorax. 1961 Jun;16(2):176-9. doi: 10.1136/thx.16.2.176.
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