通过富勒土法或酶法测定肌酐，对小鼠尿液适用，但对小鼠血浆不适用。

Creatinine assay by the fuller's earth procedure or by enzymatic determination is adequate for urine but not plasma of mice.

Meyer M H, Smith S, Meyer R A

Baxter Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232-2861.

Comp Biochem Physiol B. 1993 Nov;106(3):685-9. doi: 10.1016/0305-0491(93)90150-4.

Abstract

Mouse plasma creatinine measured by fuller's earth (15.1 +/- 0.5(41) microM, mean +/- SE) exceeded the same pools read by HPLC [7.5 +/- 0.2(41); P < 0.001]. 2. For mouse urine, the fuller's earth method (3.26 +/- 0.21 mM) slightly exceeded the HPLC method (2.65 +/- 0.18; P < 0.001) with good correlation (r = 0.915, P < 0.001). 3. An enzymatic method (creatinine PAP, Boehringer-Mannheim Diagnostics) for plasma creatinine (5.2 +/- 0.3 microM) was equivalent to HPLC (6.1 +/- 0.1, NS) but with poor correlation (r = 0.23, NS). 4. Creatinine in the urine agreed closely between HPLC (2.84 +/- 0.12 mM) and the enzymatic procedure (2.95 +/- 0.17, NS) with good correlation (r = 0.845, P < 0.001).

摘要

用富勒土法测得的小鼠血浆肌酐（15.1±0.5(41)微摩尔，均值±标准误）超过了用高效液相色谱法测得的同一组数据[7.5±0.2(41)；P<0.001]。2. 对于小鼠尿液，富勒土法（3.26±0.21毫摩尔）略高于高效液相色谱法（2.65±0.18；P<0.001），且相关性良好（r = 0.915，P<0.001）。3. 一种用于血浆肌酐的酶法（肌酐PAP，勃林格殷格翰诊断公司）测得的值为（5.2±0.3微摩尔），与高效液相色谱法（6.1±0.1，无显著差异）相当，但相关性较差（r = 0.23，无显著差异）。4. 尿液中的肌酐在高效液相色谱法（2.84±0.12毫摩尔）和酶法（2.95±0.17，无显著差异）之间吻合度高，且相关性良好（r = 0.845，P<0.001）。