Klughammer C, Schreiber U
Julius-von-Sachs-Institut, Lehrstuhl Botanik I, Universität Würzburg, Germany.
FEBS Lett. 1993 Dec 28;336(3):491-5. doi: 10.1016/0014-5793(93)80862-o.
Valinomycin/K+ is shown to selectively interact at sub-micromolar concentrations with the cytochrome bf complex in thylakoid membranes, inducing a red shift of the ferrohaem b absorbance alpha-band, a slow down of post-illumination b-reoxidation and a corresponding increase of b-reduction level in continuous light. These effects of valinomycin/K+ are not related to its field dissipating action, as they are not affected by nonactin. Presence of K+ is required. Phenomenologically the valinomycin/K+ effects are similar to those caused by 10 times higher MOA stilbene nonactin. Presence of K+ is required. Phenomenologically the valinomycin/K+ effects are similar to those caused by 10 times higher MOA stilbene concentrations. However, synergism is observed between the two inhibitors, suggesting different modes of action. When both inhibitors are combined more than one haem b can be reduced by illumination.
缬氨霉素/钾离子在亚微摩尔浓度下与类囊体膜中的细胞色素bf复合物发生选择性相互作用，导致亚铁血红素b吸收α带出现红移，光照后b再氧化减缓，持续光照下b还原水平相应增加。缬氨霉素/钾离子的这些效应与其场消散作用无关，因为它们不受尼日利亚菌素影响。钾离子的存在是必需的。从现象学角度来看，缬氨霉素/钾离子的效应与10倍摩尔作用浓度更高的二苯乙烯尼日利亚菌素所引起的效应相似。钾离子的存在是必需的。从现象学角度来看，缬氨霉素/钾离子的效应与10倍摩尔作用浓度更高的二苯乙烯浓度所引起的效应相似。然而，在这两种抑制剂之间观察到协同作用，表明它们的作用模式不同。当两种抑制剂联合使用时，光照可使不止一个血红素b被还原。
