Heimann S, Klughammer C, Schreiber U
Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Universität Würzburg, Germany.
FEBS Lett. 1998 Apr 10;426(1):126-30. doi: 10.1016/s0014-5793(98)00326-3.
Under normal physiological conditions the state of the cyt bf complex is characterized by rapid reoxidation kinetics of cyt b-563 following flash-illumination. It is known that these kinetics are dramatically slowed down under oxidizing conditions. Here we show that this slow-down of cyt b-563 oxidation is the consequence of a relatively slow (half-time of several minutes) transformation of the cyt bf complex into a distinctly different state (termed state-s). Reversal to the normal state requires strong reductive treatment or light-induced electron transport. The results are in line with a recent model of functional cyt bf dimers [Cramer et al., Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 (1996), 477-5081, if it is assumed that state-s reflects the monomeric state of the bf complex.
在正常生理条件下，细胞色素bf复合体的状态以闪光照射后细胞色素b-563的快速再氧化动力学为特征。已知在氧化条件下这些动力学显著减慢。在此我们表明，细胞色素b-563氧化的这种减慢是细胞色素bf复合体向明显不同状态（称为状态-s）相对缓慢（半衰期为几分钟）转变的结果。恢复到正常状态需要强烈的还原处理或光诱导电子传递。如果假设状态-s反映了bf复合体的单体状态，这些结果与最近关于功能性细胞色素bf二聚体的模型[克莱默等人，《植物生理学和植物分子生物学年度评论》47 (1996)，477 - 5081]一致。
