Elhai J
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824.
FEMS Microbiol Lett. 1993 Dec 1;114(2):179-84. doi: 10.1111/j.1574-6968.1993.tb06570.x.
The strengths of several promoters were assessed in the cyanobacterium Anabaena PCC 7120 by fusing them to luxAB, encoding bacterial luciferase. Two promoters, Ptac and PpsbA, with sequences nearly identical to consensus Escherichia coli sigma 70 promoters, gave as high or higher expression than the strong Anabaena promoter, Prbc. Pnpt, the natural promoter driving expression of the kanamycin-resistance determinant from Tn5, was poorly expressed in Anabaena. The Lac repressor partially repressed expression from Ptac, permitting regulated expression in Anabaena after induction with isopropyl thiogalactoside to a level 4-5-fold higher than without inducer.
通过将几个启动子与编码细菌荧光素酶的luxAB融合，在蓝藻鱼腥藻PCC 7120中评估了它们的强度。两个启动子Ptac和PpsbA，其序列与大肠杆菌σ70启动子的共有序列几乎相同，其表达水平与鱼腥藻的强启动子Prbc相当或更高。驱动来自Tn5的卡那霉素抗性决定簇表达的天然启动子Pnpt，在鱼腥藻中表达不佳。Lac阻遏物部分抑制了Ptac的表达，在用异丙基硫代半乳糖苷诱导后，可在鱼腥藻中实现调控表达，其表达水平比未诱导时高4至5倍。
