Jansson P A, Larsson A, Smith U, Lönnroth P
Department of Internal Medicine, University of Göteborg, Sahlgren's Hospital, Sweden.
J Clin Invest. 1994 Jan;93(1):240-6. doi: 10.1172/JCI116951.
Lactate concentration in the subcutaneous interstitial fluid and adipose tissue blood flow (ATBF, ml/100 g.min) were simultaneously measured with the microdialysis technique combined with 133Xe clearance in the abdominal and femoral subcutaneous adipose tissue in nine lean and nine obese men. The studies were performed both in the postabsorptive state and 2 h after an oral glucose load and the results compared to the lactate levels in arterialized venous plasma. After an overnight's fast, arterial lactate was 738 +/- 49 and 894 +/- 69 microM (mean +/- SE) (P < 0.05) in the lean and obese subjects, respectively. The interstitial lactate levels were significantly higher than blood lactate in both subject groups without any regional differences. Abdominal and femoral ATBF was 3.2 +/- 0.6 vs. 2.8 +/- 0.4 and 1.7 +/- 0.3 vs. 2.4 +/- 0.4 ml/100 g.min (P < 0.05) in lean and obese subjects, respectively. Mean apparent lactate release from the abdominal vs. femoral adipose tissue in the fasting state was 10.5 +/- 3.1 vs. 8.6 +/- 2.3 and 6.0 +/- 2.3 vs. 8.5 +/- 2.3 mumol/kg.min (NS) in lean and obese subjects, respectively. Both plasma and interstitial lactate levels increased significantly after an oral glucose load in both subject groups. However, apparent lactate release increased significantly only in the lean group. It is concluded that subcutaneous adipose tissue is a significant source of whole-body lactate release in the postabsorptive state and that this is further enhanced in obese subjects due to their large adipose mass.
采用微透析技术结合133Xe清除法，同时测定了9名瘦男性和9名肥胖男性腹部和股部皮下脂肪组织中皮下组织间液的乳酸浓度和脂肪组织血流量（ATBF，ml/100g·min）。研究分别在吸收后状态和口服葡萄糖负荷后2小时进行，并将结果与动脉化静脉血浆中的乳酸水平进行比较。禁食过夜后，瘦受试者和肥胖受试者的动脉乳酸水平分别为738±49和894±69μM（平均值±标准误）（P<0.05）。在两个受试者组中，组织间液乳酸水平均显著高于血乳酸水平，且无任何区域差异。瘦受试者和肥胖受试者的腹部和股部ATBF分别为3.2±0.6 vs. 2.8±0.4和1.7±0.3 vs. 2.4±0.4 ml/100g·min（P<0.05）。在禁食状态下，瘦受试者和肥胖受试者腹部与股部脂肪组织的平均表观乳酸释放量分别为10.5±3.1 vs. 8.6±2.3和6.0±2.3 vs. 8.5±2.3μmol/kg·min（无显著性差异）。在两个受试者组中，口服葡萄糖负荷后血浆和组织间液乳酸水平均显著升高。然而，仅在瘦组中表观乳酸释放量显著增加。结论是，皮下脂肪组织是吸收后状态下全身乳酸释放的重要来源，并且由于肥胖受试者脂肪量较大，这一来源在肥胖受试者中进一步增强。
