神经母细胞瘤中DNA倍体和增殖活性预后价值的回顾性研究。
Retrospective study of prognostic value of DNA ploidy and proliferative activity in neuroblastoma.
作者信息
Huddart S N, Muir K R, Parkes S E, Mann J R, Stevens M C, Raafat F, Smith K
机构信息
Royal Manchester Children's Hospital, Pendlebury, Manchester.
出版信息
J Clin Pathol. 1993 Dec;46(12):1101-4. doi: 10.1136/jcp.46.12.1101.
AIM
To assess the prognostic value of age and stage at diagnosis, site of primary tumour, cell ploidy and N-myc copy number in children with neuroblastoma.
METHODS
Flow cytometry was used to determine the cellular DNA content of paraffin wax embedded archival material from 69 cases of neuroblastoma and was successful in 52.
RESULTS
The age, stage, and survival distribution of the sampled cases was not significantly different from that in a larger population based series. There were seven diploid ("non-aneuploid") and 45 aneuploid (including two tetraploid and four triploid) tumours. The 10 year survival was significantly better for cases of aneuploid rather than diploid tumours (p < 0.05). An important new finding was that 10 year survival was also significantly better for tumours with a low percentage of cells in S phase (p < 0.03).
CONCLUSION
The percentage of cells in S phase, a measure of the proliferative activity of the tumour, correlated with prognosis in neuroblastoma. This should be measured with other biological features of the disease, such as N-myc copy number, when prognostic indicators are being assessed.
目的
评估神经母细胞瘤患儿诊断时的年龄和分期、原发肿瘤部位、细胞倍体及N - myc拷贝数的预后价值。
方法
采用流式细胞术测定69例神经母细胞瘤石蜡包埋存档材料的细胞DNA含量，52例成功测定。
结果
抽样病例的年龄、分期及生存分布与基于更大人群的系列研究无显著差异。有7例二倍体（“非非整倍体”）肿瘤和45例非整倍体肿瘤（包括2例四倍体和4例三倍体）。非整倍体肿瘤病例的10年生存率显著高于二倍体肿瘤（p < 0.05）。一个重要的新发现是，S期细胞百分比低的肿瘤10年生存率也显著更高（p < 0.03）。
结论
S期细胞百分比作为肿瘤增殖活性的指标，与神经母细胞瘤的预后相关。在评估预后指标时，应将其与该疾病的其他生物学特征如N - myc拷贝数一起测定。
Zotero 插件