Hasebe M, Ito M, Kofuji R, Ueda K, Iwatsuki K
Botanical Gardens, University of Tokyo, Japan.
J Mol Evol. 1993 Nov;37(5):476-82. doi: 10.1007/BF00160428.
Part of the large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) gene (rbcL) was sequenced from three fern species: Adiantum capillus-veneris, Botrypus strictus, and Osmunda cinnamomea var. fokiensis. This region included 1,333 base pairs, about 90% of the gene. Maximum likelihood analysis of the deduced amino acid sequences indicated that (1) Botrypus (Ophioglossaceae) clustered monophyletically with other ferns (Adiantum, Angiopteris, Osmunda); the closest relative to Botrypus among the three species was Osmunda, which did not support the hypothesis that the Ophioglossaceae are linked to the progymnosperm-seed plant lineage. (2) Eusporangiate ferns containing Botrypus (Ophioglossaceae) and Angiopteris (Marattiaceae) were a paraphyletic group. (3) Seed plants and the four fern species examined formed a monophyletic group, but ferns and bryophytes (liverwort) did not. Variations in rates of substitution for synonymous and nonsynonymous codons were found in fern lineages.
对三种蕨类植物——铁线蕨、阴地蕨和福建紫萁的1,5 - 二磷酸核酮糖羧化酶/加氧酶（rubisco）基因（rbcL）的大亚基部分进行了测序。该区域包含1333个碱基对，约占该基因的90%。对推导的氨基酸序列进行的最大似然分析表明：（1）阴地蕨科的阴地蕨与其他蕨类植物（铁线蕨、观音座莲、紫萁）单系聚类；在这三个物种中，与阴地蕨亲缘关系最近的是紫萁，这并不支持阴地蕨科与前裸子植物 - 种子植物谱系相关的假说。（2）包含阴地蕨（阴地蕨科）和观音座莲（合囊蕨科）的厚囊蕨类是一个并系类群。（3）种子植物和所研究的四种蕨类植物形成一个单系类群，但蕨类植物和苔藓植物（地钱）并非如此。在蕨类植物谱系中发现了同义密码子和非同义密码子替换率的差异。
