Hirai Y, Haque M, Yokota K, Oguma K
Okayama University Medical School, Department of Bacteriology.
Nihon Rinsho. 1993 Dec;51(12):3094-101.
In H. pylori, we examined the composition of total lipids. In thin-layer chromatography, three kinds of glycolipids were detected, which were named as G-1, G-2 and G-3. G-3 contained phosphates in addition to sugars. Total lipids consisted of 6.0% neutral lipids (weight %), 20.6% glycolipids (G-1, G-2) and 73.4% phospholipids (including G-3). Total glycolipids were approximately 25% of total lipids. After methanolysis, cholesterol was detected in the hexane extractable fraction and glucose was detected by GLC in the methanol fraction after trimethylsilylation, in all glycolipids. Therefore, these glycolipids are cholesteryl glycosides. Cholesteryl glycosides are found very rarely in bacteria, and have been detected only in a few species of mycoplasmas and spirochetes.
在幽门螺杆菌中，我们检测了总脂质的组成。在薄层层析中，检测到三种糖脂，分别命名为G - 1、G - 2和G - 3。G - 3除了含有糖之外还含有磷酸盐。总脂质由6.0%的中性脂质（重量百分比）、20.6%的糖脂（G - 1、G - 2）和73.4%的磷脂（包括G - 3）组成。总糖脂约占总脂质的25%。甲醇解后，在己烷可萃取部分中检测到胆固醇，在所有糖脂经三甲基硅烷化后的甲醇部分中通过气相色谱法检测到葡萄糖。因此，这些糖脂是胆固醇糖苷。胆固醇糖苷在细菌中非常罕见，仅在少数支原体和螺旋体物种中被检测到。
