Sheldrake R F, Romalis L F, Saunders M M
Elizabeth Macarthur Agricultural Institute, Camden, New South Wales, Australia.
Res Vet Sci. 1993 Nov;55(3):371-6. doi: 10.1016/0034-5288(93)90110-2.
Pigs were immunised intraperitoneally when six weeks old and again at about 10 weeks old with killed Mycoplasma hyopneumoniae antigen prepared in an oil adjuvant. The pigs were challenged with live M hyopneumoniae (Beaufort strain) at between 11 and 15 weeks old. Antigen specific antibody levels for both IgG and IgA classes in serum and respiratory tract secretion were monitored over time. In serum anti-M hyopneumoniae antibody was detected shortly after the second intraperitoneal vaccination and was largely IgG. In respiratory tract secretion the response was observed after challenge, and was primarily IgA. Anti-M hyopneumoniae antibody-containing cells and their immunoglobulin class specificity were monitored in lung and tracheal lamina propria. In lung the majority of anti-M hyopneumoniae-containing cells were IgG, whereas in the tracheal lamina propria the majority were IgA. These results are discussed in terms of the use of intraperitoneal vaccination for the control of M hyopneumoniae infection.
六周龄的猪通过腹腔注射，在大约十周龄时再次用在油佐剂中制备的灭活猪肺炎支原体抗原进行免疫。这些猪在11至15周龄时用活的猪肺炎支原体（博福特菌株）进行攻毒。随时间监测血清和呼吸道分泌物中IgG和IgA两类抗原特异性抗体水平。在血清中，第二次腹腔注射疫苗后不久就检测到抗猪肺炎支原体抗体，且主要是IgG。在呼吸道分泌物中，攻毒后观察到反应，且主要是IgA。在肺和气管固有层中监测含抗猪肺炎支原体抗体的细胞及其免疫球蛋白类别特异性。在肺中，大多数含抗猪肺炎支原体的细胞是IgG，而在气管固有层中大多数是IgA。就使用腹腔注射疫苗控制猪肺炎支原体感染对这些结果进行了讨论。
