针对EB病毒反式激活因子ZEBRA抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for antibodies to ZEBRA, an Epstein-Barr trans-activator.
作者信息
Maréchal V, Meyohas M C, Joab I, Gaha S, Giot J F, Sergeant A, Nicolas J C
机构信息
Laboratoire de Microbiologie, Hôpital Rothschild, Paris, France.
出版信息
Res Virol. 1993 Sep-Oct;144(5):397-404. doi: 10.1016/s0923-2516(06)80055-5.
Patients with AIDS and AIDS-related complex often show symptoms of Epstein-Barr virus (EBV) reactivation. Several EBV-encoded trans-acting factors activate the EBV lytic cycle, and one, called ZEBRA (BamHI Z EBV replication activator), controls the switch of EBV from a latent to a productive cycle. We describe here a simple ELISA test using a bacterially expressed ZEBRA protein as antigen. Utilizing this technique, we evaluated the humoral response to ZEBRA antigen in 38 patients with nasopharyngeal carcinoma and 134 subjects with an asymptomatic HIV 1 infection. The control group consisted of 40 healthy adult blood donors.
艾滋病患者及艾滋病相关综合征患者常表现出EB病毒（EBV）再激活的症状。几种EBV编码的反式作用因子可激活EBV裂解周期，其中一种名为ZEBRA（BamHI Z EBV复制激活因子）的因子控制着EBV从潜伏周期向增殖周期的转变。我们在此描述一种简单的酶联免疫吸附测定（ELISA）试验，该试验使用细菌表达的ZEBRA蛋白作为抗原。利用这项技术，我们评估了38例鼻咽癌患者和134例无症状HIV-1感染受试者对ZEBRA抗原的体液免疫反应。对照组由40名健康成年献血者组成。
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