Miltenburg G A, Wensing T, Breukink H J, Marx J J
Department of Large Animal Medicine and Nutrition, Faculty of Veterinary Medicine, State University of Utrecht, The Netherlands.
Vet Res Commun. 1993;17(3):209-17. doi: 10.1007/BF01839169.
A method for studying iron absorption in humans was adapted to veal calves. Three 10-week-old calves with moderate (calves 1 and 2) or severe (calf 3) iron deficiency were given an abomasal injection of 59Fe and 51Cr and all their faeces were collected over 15 days in order to measure mucosal uptake, mucosal transfer and retention of iron. The mucosal uptake was 62.2, 53.4 and 71.8% in calves 1, 2 and 3, respectively. The iron retention measured 14 days after administration of the test dose was 57.4, 52.3 and 56.4% in calves 1, 2 and 3, respectively. Maximal plasma activity was found in all three calves between 1 1/2 and 2 h after injection of the test dose. The plasma activity decreased rapidly, with a slight increase between the 5th and the 10th hour. After 21 h, less than 0.25% of the injected dose was still present in 1 litre of plasma. Not all the 51Cr was recovered in the faeces. No 59Fe was found in the urine but some 51Cr could be detected. The results of this study show that the method described is useful for measuring the different steps of iron absorption in iron-deficient veal calves.
一种研究人体铁吸收的方法被应用于小牛肉牛犊。三只10周龄、患有中度（牛犊1和2）或重度（牛犊3）缺铁的小牛经皱胃注射59Fe和51Cr，并在15天内收集它们的所有粪便，以测量铁的黏膜摄取、黏膜转运和潴留情况。牛犊1、2和3的黏膜摄取率分别为62.2%、53.4%和71.8%。给药后14天测得的铁潴留率在牛犊1、2和3中分别为57.4%、52.3%和56.4%。在注射测试剂量后1.5至2小时，所有三只小牛均出现最大血浆活性。血浆活性迅速下降，在第5至第10小时略有上升。21小时后，每升血浆中仍存在的注射剂量不到0.25%。并非所有的51Cr都在粪便中回收。尿液中未发现59Fe，但可检测到一些51Cr。本研究结果表明，所描述的方法可用于测量缺铁小牛肉牛犊铁吸收的不同步骤。
