Rosenzweig M, Clark D P, Gaulton G N
Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, Philadelphia 19104.
AIDS. 1993 Dec;7(12):1601-5. doi: 10.1097/00002030-199312000-00009.
To determine the impact of HIV-1 infection on thymocyte development, and the role of thymic infection on the pathogenesis of neonatal HIV-1 infection.
The consequences of thymic infection by HIV-1 were examined by comparative histologic and molecular analyses of an asymptomatic, HIV-1-seropositive 3-day-old subject, versus age- and treatment-matched controls. The presence of replicating virus was established by in situ hybridization with specific molecular probes to HIV-1. The distribution of thymocyte subsets was determined by quantitative flow cytometry following staining with antibodies to CD4 and CD8 cell surface proteins.
The results show clear evidence of severe thymic involution, HIV-1 infection of thymocytes, and selective depletion of thymocyte subpopulations. The consequences of HIV-1 infection were a marked depletion of CD3+CD4+ CD8hi and CD3+CD4+CD8- cells. The phenotype of the residual thymic lymphoid population was predominantly that of immature CD3-CD4-CD8- double negative and CD3+CD4+CD8lo cells.
Changes in the distribution of thymocyte subsets suggests a role for thymic involvement in the pathogenesis of HIV-1 infection in neonates.
确定人类免疫缺陷病毒1型（HIV-1）感染对胸腺细胞发育的影响，以及胸腺感染在新生儿HIV-1感染发病机制中的作用。
通过对一名无症状、HIV-1血清阳性的3日龄婴儿与年龄及治疗相匹配的对照进行比较组织学和分子分析，研究HIV-1胸腺感染的后果。通过用针对HIV-1的特异性分子探针进行原位杂交确定复制病毒的存在。在用抗CD4和CD8细胞表面蛋白抗体染色后，通过定量流式细胞术确定胸腺细胞亚群的分布。
结果显示有明确证据表明存在严重胸腺萎缩、胸腺细胞的HIV-1感染以及胸腺细胞亚群的选择性耗竭。HIV-1感染的后果是CD3+CD4+CD8hi和CD3+CD4+CD8-细胞显著减少。残余胸腺淋巴样群体的表型主要是未成熟的CD3-CD4-CD8-双阴性和CD3+CD4+CD8lo细胞。
胸腺细胞亚群分布的变化表明胸腺参与新生儿HIV-1感染发病机制。
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