Hashizume H, Iwatsuki K, Takigawa M
Department of Dermatology, Hamamatsu University School of Medicine, Japan.
Br J Dermatol. 1993 Dec;129(6):739-43. doi: 10.1111/j.1365-2133.1993.tb03345.x.
The serum of a 34-year-old woman with the Hallopeau type of pemphigus vegetans (PVg) contained antibodies against a 130-kDa polypeptide in human epidermal lysates, as revealed by Western blot analysis. The serum strongly fixed complement in vitro, and the PVg lesional skin contained a predominance of complement-fixing IgG2 and IgG4. Although the antigens reactive with sera from PVg and pemphigus vulgaris were the same, strong fixation of complement by PVg antibodies, due to the presence of complement-dependent IgG subclasses, and subsequent in situ activation of complement, might explain the marked infiltration of neutrophils and eosinophils in PVg.
蛋白质印迹分析显示，一名患有Hallopeau型增殖性天疱疮（PVg）的34岁女性血清中含有针对人表皮裂解物中一种130 kDa多肽的抗体。该血清在体外能强烈固定补体，PVg皮损皮肤中主要含有可固定补体的IgG2和IgG4。尽管与PVg和寻常型天疱疮血清反应的抗原相同，但由于存在补体依赖性IgG亚类，PVg抗体对补体的强烈固定以及随后补体的原位激活，可能解释了PVg中嗜中性粒细胞和嗜酸性粒细胞的显著浸润。
