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粟酒裂殖酵母II类果糖-1,6-二磷酸醛缩酶基因同源物的分子克隆与核苷酸测序

Molecular cloning and nucleotide sequencing of Schizosaccharomyces pombe homologue of the class II fructose-1,6-bisphosphate aldolase gene.

作者信息

Mutoh N, Hayashi Y

机构信息

Department of Genetics, Institute for Developmental Research, Aichi, Japan.

出版信息

Biochim Biophys Acta. 1994 Jan 4;1183(3):550-2. doi: 10.1016/0005-2728(94)90084-1.

Abstract

DNA fragment containing Schizosaccharomyces pombe homologue of the class II fructose-1,6-bisphosphate aldolase gene was cloned and sequenced. A long open reading frame, which encodes a polypeptide of 358 amino acid residues, was found in the sequence. Amino acid sequence deduced from the nucleotide sequence is 63% homologous to the amino acid sequence of the enzyme of Saccharomyces cerevisiae. Northern blot analysis revealed that 1.3 kb poly(A)+ RNA is transcribed from this DNA sequence.

摘要

含有粟酒裂殖酵母II类果糖-1,6-二磷酸醛缩酶基因同源物的DNA片段被克隆并测序。在该序列中发现了一个长开放阅读框,其编码一个由358个氨基酸残基组成的多肽。从核苷酸序列推导的氨基酸序列与酿酒酵母该酶的氨基酸序列有63%的同源性。Northern印迹分析表明,1.3 kb的聚腺苷酸化RNA是从该DNA序列转录而来的。

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