Oren S L, Swenson S L, Kinker D R, Hill H T, Hu H L, Zimmerman J
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011.
J Vet Diagn Invest. 1993 Oct;5(4):529-33. doi: 10.1177/104063879300500405.
Six enzyme-linked immunosorbent assays, a latex agglutination test, and the standard microtitration serum virus neutralization test were compared for their ability to detect antibodies against pseudorabies virus (PRV) during the early stages of infection. Thirty-five pigs were infected intranasally with 10(5)-10(7) TCID50 of either the Iowa 4892 pneumotropic or the Becker strain of PRV. Blood samples were drawn from experimentally inoculated animals on days 4-10, 14, and 21 postchallenge. Test sensitivity estimates and comparisons among tests were made for each sampling day over the 21-day monitoring period. Results of this study demonstrated differences among tests in 1) the time from inoculation to initial antibody detection, 2) the time to detect > or = 95% of the infected pigs, and 3) the time from initial antibody detection to determination of > or = 95% as positive. By day 10 postchallenge, no statistically significant difference in diagnostic sensitivity was observed among the 8 tests compared in the study.
对六种酶联免疫吸附测定法、一种乳胶凝集试验和标准微量滴定血清病毒中和试验检测感染初期抗伪狂犬病病毒（PRV）抗体的能力进行了比较。35头猪经鼻内接种10(5)-10(7) TCID50的爱荷华4892嗜肺型或PRV贝克尔株。在攻毒后第4-10天、14天和21天从实验接种的动物采集血样。在21天监测期内，对每个采样日的试验敏感性估计值及试验间比较进行了分析。本研究结果表明，各试验在以下方面存在差异：1）从接种到首次检测到抗体的时间；2）检测到≥95%感染猪的时间；3）从首次检测到抗体到确定≥95%为阳性的时间。攻毒后第10天，研究中比较的8种试验在诊断敏感性上未观察到统计学显著差异。
