Rodgers S J, Karges S L, Saliki J T
Oklahoma Animal Disease Diagnostic Laboratory, Oklahoma State University, Stillwater 74078, USA.
J Vet Diagn Invest. 1996 Apr;8(2):168-71. doi: 10.1177/104063879600800205.
Rapid screening of large numbers of swine sera for antibody is an essential element in the current eradication program for pseudorabies in the United States. We evaluated a recently introduced commercial semiautomated latex agglutination test (LAT) kit for pseudorabies antibody. A total of 1,191 swine sera were tested using the new procedure and 3 established tests: the manual LAT, the serum neutralization test (SNT), and an enzyme-linked immunosorbent assay (ELISA). There was close agreement among results of semiautomated LAT, the manual LAT, and ELISA but less agreement between semiautomated LAT and SNT. Overall, the sensitivities of the 4 tests were as follows: semiautomated LAT = manual LAT > ELISA > SNT. For 74 samples of known pseudorabies antibody status, the overall specificities were as follows: semi-automated LAT = manual LAT = SNT > ELISA. Because of its relative insensitivity, the SNT should no longer be considered the official "gold" standard for pseudorabies testing in the on-going eradication program. However, because no single test was perfect for all samples, a scheme including 3 tests in the following sequence is recommended: 1) screening using semiautomated LAT or ELISA and 2) confirmation testing of positives by manual LAT and SNT, with any sample that tests positive by any 2 tests being regarded as true positive.
快速筛查大量猪血清中的抗体是美国当前伪狂犬病根除计划的一项基本要素。我们评估了一种最近推出的用于检测伪狂犬病抗体的商业半自动乳胶凝集试验（LAT）试剂盒。使用新方法和3种既定检测方法对总共1191份猪血清进行了检测：手工LAT、血清中和试验（SNT）和酶联免疫吸附测定（ELISA）。半自动LAT、手工LAT和ELISA的检测结果之间一致性良好，但半自动LAT与SNT之间的一致性较差。总体而言，4种检测方法的敏感性如下：半自动LAT = 手工LAT > ELISA > SNT。对于74份已知伪狂犬病抗体状态的样本，总体特异性如下：半自动LAT = 手工LAT = SNT > ELISA。由于其相对不敏感，在当前的根除计划中，SNT不应再被视为伪狂犬病检测的官方“金”标准。然而，由于没有一种检测方法对所有样本都完美适用，建议采用以下顺序包含3种检测方法的方案：1）使用半自动LAT或ELISA进行筛查，2）通过手工LAT和SNT对阳性样本进行确认检测，任何通过任意2种检测方法呈阳性的样本都被视为真阳性。
