Kinker D R, Swenson S L, Wu L L, Zimmerman J J
Veterinary Diagnostic Laboratory, Iowa State University, Ames 50011, USA.
Vet Microbiol. 1997 Apr;55(1-4):99-106. doi: 10.1016/s0378-1135(96)01308-9.
Two enzyme-linked immunosorbent assays (ELISAs) and a particle concentration fluorescence immunoassay (PCFIA) were compared for their ability to detect antibodies against pseudorabies virus (Aujeszky's disease virus) glycoprotein E (gE) in the early stages of infection in pigs previously vaccinated with gE-deleted pseudorabies vaccines. Seventy pigs were included in the study. Five groups of 6 pigs each were vaccinated with one of 5 different pseudorabies virus (PRV) gE-deleted vaccines, and subsequently infected intranasally with 10(5.6) TCID50 of the Iowa 4892 pneumotropic strain of PRV. This entire procedure was repeated using 10(4.6) TCID50 of the Rice strain of PRV. Five unvaccinated control pigs were also challenged with each virus strain. Three control pigs died before seroconverting, leaving 67 pigs for comparison. Blood samples were drawn from experimentally inoculated pigs on the day of vaccination, the day of challenge, and on 4-10, 14, and 21 days postchallenge (DPC). Serology test sensitivity estimates and comparisons among tests were made for each sampling day. Results of this study demonstrated differences among the tests in the time from inoculation to initial antibody detection, and the time to detect 50% and 75% of the infected pigs. The average time until first detection of pigs as seropositive for gE antibodies by PCFIA was 7.5 DPC. The blocking ELISA detected pigs as seropositive an average of 8.8 DPC, and the indirect ELISA first detected gE antibodies by 9.3 DPC. Fifty percent of the pigs were detected as seropositive by days 7, 8, and 9 for the PCFIA, blocking ELISA, and indirect ELISA, respectively. Similarly, 75% of the pigs were detected as seropositive by days 8, 9, and 10 for the PCFIA, blocking ELISA, and indirect ELISA, respectively. All pigs were detected as seropositive by 14 DPC for all 3 tests.
对两种酶联免疫吸附测定（ELISA）和一种颗粒浓度荧光免疫测定（PCFIA）进行了比较，以评估它们在检测先前接种过缺失gE的伪狂犬病疫苗的猪感染早期针对伪狂犬病病毒（奥耶斯基氏病病毒）糖蛋白E（gE）抗体的能力。该研究共纳入70头猪。将70头猪分为5组，每组6头，分别接种5种不同的缺失伪狂犬病病毒（PRV）gE的疫苗之一，随后经鼻内接种10(5.6) TCID50的爱荷华4892嗜肺性PRV毒株。使用10(4.6) TCID50的PRV稻株重复整个过程。还对每组5头未接种疫苗的对照猪用每种病毒株进行攻毒。3头对照猪在血清转化前死亡，剩余67头猪用于比较。在接种疫苗当天、攻毒当天以及攻毒后4 - 10天、14天和21天（DPC）从实验接种的猪采集血样。对每个采样日进行血清学检测敏感性估计和检测方法之间的比较。本研究结果表明，在从接种到首次检测到抗体的时间以及检测到50%和75%感染猪的时间方面，各检测方法存在差异。通过PCFIA首次检测到猪gE抗体血清阳性的平均时间为7.5 DPC。阻断ELISA检测到猪血清阳性的平均时间为8.8 DPC，间接ELISA首次检测到gE抗体的时间为9.3 DPC。PCFIA、阻断ELISA和间接ELISA分别在第7天、第8天和第9天检测到50%的猪血清阳性。同样，PCFIA、阻断ELISA和间接ELISA分别在第8天、第9天和第10天检测到75%的猪血清阳性。在攻毒后14 DPC时，所有3种检测方法均检测到所有猪血清阳性。
